ArticleJournal of virology2002
RU5 of Mason-Pfizer monkey virus 5' long terminal repeat enhances cytoplasmic expression of human immunodeficiency virus type 1 gag-pol and nonviral reporter RNA.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 33 citations in OpenAlex.
- Review
- Anomalous HIV-1 RNA, How Cap-Methylation Segregates Viral Transcripts by Form and Function.Viruses · 2022Review
- HIV-1 hypermethylated guanosine cap licenses specialized translation unaffected by mTOR.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- RNA Helicase A Regulates the Replication of RNA Viruses.Viruses · 2021Review
- The U3 and Env Proteins of Jaagsiekte Sheep Retrovirus and Enzootic Nasal Tumor Virus Both Contribute to Tissue Tropism.Viruses · 2019Article
- Identification of conserved, primary sequence motifs that direct retrovirus RNA fate.Nucleic acids research · 2018Article
- The U3 region of Moloney murine leukemia virus contains position-independent cis-acting sequences involved in the nuclear export of full-length viral transcripts.The Journal of biological chemistry · 2014Article
- Gammaretrovirus mRNA expression is mediated by a novel, bipartite post-transcriptional regulatory element.Nucleic acids research · 2014Article
- The 0.3-kb fragment containing the R-U5-5'leader sequence of Friend murine leukemia virus influences the level of protein expression from spliced mRNA.Virology journal · 2013Article
- A novel role of RNA helicase A in regulation of translation of type I collagen mRNAs.RNA (New York, N.Y.) · 2012Article
- Translation of MMTV Gag requires nuclear events involving splicing motifs in addition to the viral Rem protein and RmRE.Retrovirology · 2012Article
- Thriving under stress: selective translation of HIV-1 structural protein mRNA during Vpr-mediated impairment of eIF4E translation activity.PLoS pathogens · 2012Article
- Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes.Nucleic acids research · 2011Article
- Features of double-stranded RNA-binding domains of RNA helicase A are necessary for selective recognition and translation of complex mRNAs.The Journal of biological chemistry · 2011Article
- Coordinate roles of Gag and RNA helicase A in promoting the annealing of formula to HIV-1 RNA.Journal of virology · 2011Article
- RNA helicase A modulates translation of HIV-1 and infectivity of progeny virions.Nucleic acids research · 2010Article
- Jaagsiekte sheep retrovirus encodes a regulatory factor, Rej, required for synthesis of Gag protein.Journal of virology · 2009Article
- The signal peptide of a simple retrovirus envelope functions as a posttranscriptional regulator of viral gene expression.Journal of virology · 2009Article
- Rev and Rex proteins of human complex retroviruses function with the MMTV Rem-responsive element.Retrovirology · 2009Article
- Mechanisms employed by retroviruses to exploit host factors for translational control of a complicated proteome.Retrovirology · 2009Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Retroviruses utilize an unspliced version of their primary transcription product as an RNA template for synthesis of viral Gag and Pol structural and enzymatic proteins. Cytoplasmic expression of the gag-pol RNA is achieved despite the lack of intron removal and the presence of a long and highly structured 5' untranslated region that inhibits efficient ribosome scanning. In this study, we have identified for the first time that the 5' long terminal repeat (LTR) of Mason-Pfizer monkey virus (MPMV) facilitates Rev/Rev-responsive element-independent expression of HIV-1 gag-pol reporter RNA. The MPMV RU5 region of the LTR is necessary and directs functional interaction with cellular posttranscriptional modulators present in human 293 and monkey COS cells but not in quail QT-6 cells and does not require any viral protein. Deletion of MPMV RU5 decreases the abundance of spliced mRNA but has little effect on cytoplasmic accumulation of unspliced gag-pol RNA despite complete elimination of detectable Gag protein production. MPMV RU5 also exerts a positive effect on the cytoplasmic expression of intronless luc RNA, and ribosomal profile analysis demonstrates that MPMV RU5 directs subcellular localization of the luc transcript to polyribosomes. Our findings have a number of similarities with those of reports on 5' terminal posttranscriptional control elements in spleen necrosis virus and human foamy virus RNA and support the model that divergent retroviruses share 5' terminal RNA elements that interact with host proteins to program retroviral RNA for productive cytoplasmic expression.
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