ArticleJournal of virology2002
Molecular genetic evidence that the hydrophobic anchors of glycoproteins E2 and E1 interact during assembly of alphaviruses.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Advances in Viroporin Function and Structure: A Comparative Analysis of Alphavirus 6K with Well-Characterized Viroporins.Viruses · 2025Review
- Cryogenic electron microscopy and tomography reveal imperfect icosahedral symmetry in alphaviruses.PNAS nexus · 2024Article
- Epidemiologic, Entomologic, and Virologic Factors of the 2014-15 Ross River Virus Outbreak, Queensland, Australia.Emerging infectious diseases · 2019Article
- Review
- Characterization of chikungunya virus-like particles.PloS one · 2014Article
- Functional characterization of the alphavirus TF protein.Journal of virology · 2013Article
- Alphavirus Entry and Membrane Fusion.Viruses · 2010Article
- A structural and functional perspective of alphavirus replication and assembly.Future microbiology · 2009Review
- Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins.Journal of virology · 2007Article
- Phylogenetic analysis of Buggy Creek virus: evidence for multiple clades in the Western Great Plains, United States of America.Applied and environmental microbiology · 2006Article
- Mapping the structure and function of the E1 and E2 glycoproteins in alphaviruses.Structure (London, England : 1993) · 2006Article
- Deletions in the transmembrane domain of a sindbis virus glycoprotein alter virus infectivity, stability, and host range.Journal of virology · 2003Article
- Interactions between the transmembrane segments of the alphavirus E1 and E2 proteins play a role in virus budding and fusion.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Chimeric alphaviruses in which the 6K and glycoprotein E1 moieties of Sindbis virus are replaced with those of Ross River virus grow very poorly, but upon passage, adapted variants arise that grow >100 times better. We have sequenced the entire domain encoding the E2, 6K, and E1 proteins of a number of these adapted variants and found that most acquired two amino acid changes, which had cumulative effects. In three independent passage series, amino acid 380 of E2, which is in the transmembrane domain, was mutated from the original isoleucine to serine in two instances and to valine once. We have now changed this residue to seven others by site-directed mutagenesis and tested the effects of these mutations on the growth of both the chimera [SIN(RRE1)] and of parental Sindbis. These results indicate that the transmembrane domains of glycoproteins E2 and E1 of alphaviruses interact in a sequence-dependent manner and that this interaction is required for efficient budding and assembly of infectious virions.
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