ArticleProceedings of the National Academy of Sciences of the United States of America2002
Expression of functional neurotransmitter receptors in Xenopus oocytes after injection of human brain membranes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 87 citations in OpenAlex.
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- Selective modulation of epileptic tissue by an adenosine ABritish journal of pharmacology · 2024Article
- The Xenopus Oocyte: A Tool for Membrane Biology.Membranes · 2023Article
- Intranasal Administration of KYCCSRK Peptide Rescues Brain Insulin Signaling Activation and Reduces Alzheimer's Disease-like Neuropathology in a Mouse Model for Down Syndrome.Antioxidants (Basel, Switzerland) · 2023Article
- GABAergic Neurotransmission in Human Tissues Is Modulated by Cannabidiol.Life (Basel, Switzerland) · 2022Article
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- The "www" ofMembranes · 2022Review
- Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies.Journal of visualized experiments : JoVE · 2022Article
- Unexpected Effect of IL-1β on the Function of GABABrain sciences · 2022Article
- Mammalian Brain CaMembranes · 2022Article
- Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation.International journal for parasitology. Drugs and drug resistance · 2020Article
- Reduced neurosteroid potentiation of GABAAnnals of clinical and translational neurology · 2020Article
- Using Xenopus oocytes in neurological disease drug discovery.Expert opinion on drug discovery · 2020Review
- Neurobiological Correlates of Alpha-Tocopherol Antiepileptogenic Effects and MicroRNA Expression Modulation in a Rat Model of Kainate-Induced Seizures.Molecular neurobiology · 2018Article
- GABAPhysiological reports · 2017Article
- Forebrain-selective AMPA-receptor antagonism guided by TARP γ-8 as an antiepileptic mechanism.Nature medicine · 2016Article
- A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays.Histochemistry and cell biology · 2016Article
- Pharmacological treatment with mirtazapine rescues cortical atrophy and respiratory deficits in MeCP2 null mice.Scientific reports · 2016Article
- GABAρ subunits confer a bicuculline-insensitive component to GFAP+ cells of cerebellum.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes.Scientific reports · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Xenopus oocyte is a very powerful tool for studies of the structure and function of membrane proteins, e.g., messenger RNA extracted from the brain and injected into oocytes leads to the synthesis and membrane incorporation of many types of functional receptors and ion channels, and membrane vesicles from Torpedo electroplaques injected into oocytes fuse with the oocyte membrane and cause the appearance of functional Torpedo acetylcholine receptors and Cl(-) channels. This approach was developed further to transplant already assembled neurotransmitter receptors from human brain cells to the plasma membrane of Xenopus oocytes. Membranes isolated from the temporal neocortex of a patient, operated for intractable epilepsy, were injected into oocytes and, within a few hours, the oocyte membrane acquired functional neurotransmitter receptors to gamma-aminobutyric acid, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, kainate, and glycine. These receptors were also expressed in the plasma membrane of oocytes injected with mRNA extracted from the temporal neocortex of the same patient. All of this makes the Xenopus oocyte a more useful model than it already is for studies of the structure and function of many human membrane proteins and opens the way to novel pathophysiological investigations of some human brain disorders.
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