ArticleThe Plant cell1997
Characterization of Post-Transcriptionally Suppressed Transgene Expression That Confers Resistance to Tobacco Etch Virus Infection in Tobacco.
Article in The Plant cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 78 citations in OpenAlex.
- Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division.Plant physiology · 2005Article
- Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco.Transgenic research · 2005Article
- Translation start sequences affect the efficiency of silencing of Agrobacterium tumefaciens T-DNA oncogenes.Plant physiology · 2003Article
- Resistance to wheat streak mosaic virus in transgenic wheat engineered with the viral coat protein gene.Transgenic research · 2002Article
- A cucumber mosaic virus (CMV) RNA 1 transgene mediates suppression of the homologous viral RNA 1 constitutively and prevents CMV entry into the phloem.Journal of virology · 2001Article
- Review
- Post-transcriptional gene silencing in plum pox virus resistant transgenic European plum containing the plum pox potyvirus coat protein gene.Transgenic research · 2001Article
- Novel features of the XRN-family in Arabidopsis: evidence that AtXRN4, one of several orthologs of nuclear Xrn2p/Rat1p, functions in the cytoplasm.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Detailed characterization of the posttranscriptional gene-silencing-related small RNA in a GUS gene-silenced tobacco.RNA (New York, N.Y.) · 2000Article
- RNA degradation and models for post-transcriptional gene-silencing.Plant molecular biology · 2000Review
- Posttranscriptional gene silencing of gn1 in tobacco triggers accumulation of truncated gn1-derived RNA species.RNA (New York, N.Y.) · 1999Article
- Expression and sequence requirements for nitrite reductase co-suppression.Plant molecular biology · 1999Article
- Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing.Transgenic research · 1999Article
- Posttranscriptional gene silencing in transgenic sugarcane. Dissection Of homology-dependent virus resistance in a monocot that has a complex polyploid genomePlant physiology · 1999Article
- Stochastic and nonstochastic post-transcriptional silencing of chitinase and beta-1,3-glucanase genes involves increased RNA turnover-possible role for ribosome-independent RNA degradation.The Plant cell · 1999Article
- Production of aberrant promoter transcripts contributes to methylation and silencing of unlinked homologous promoters in trans.The EMBO journal · 1999Article
- Arabidopsis mutants impaired in cosuppression.The Plant cell · 1998Article
- Transgenes are dispensable for the RNA degradation step of cosuppression.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Initiation and maintenance of virus-induced gene silencingThe Plant cell · 1998Article
- The DNA methylation locus DDM1 is required for maintenance of gene silencing in Arabidopsis.Genes & development · 1998Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tobacco lines expressing transgenes that encode tobacco etch virus (TEV) coat protein (CP) mRNA with or without nonsense codons give rise to TEV-resistant tissues that have reduced levels of TEV CP mRNA while maintaining high levels of transgene transcriptional activity. Two phenotypes for virus resistance in the lines containing the transgene have been described: immune (no virus infection) and recovery (initial systemic symptoms followed by gradual recovery over several weeks). Here, we show that at early times in development, immune lines are susceptible to TEV infection and accumulate full-length CP mRNA. Therefore, immune lines also exhibit meiotic resetting, as is seen in the recovery lines, providing molecular evidence for a common mechanism of gene silencing and virus resistance in both cases. We also investigated the characteristics of two sets of low molecular weight RNAs that appear only in silenced tissue. One set has nearly intact 5[prime] ends, lacks poly(A) tails, and is associated with polyribosomes; the second set contains the 3[prime] end of the mRNA. Treating silenced leaf tissue with cycloheximide resulted in decreased levels of full-length mRNA and an increase in the levels of the low molecular weight RNAs, supporting a cytoplasmic decay mechanism that does not require ongoing translation. Surprisingly, mRNA from the transgene containing nonsense codons was associated with more ribosomes than expected, possibly resulting from translation from a start codon downstream of the introduced translational stop codons. We present a hypothesis for transgene/viral RNA degradation in which RNA degradation occurs in the cytoplasm while in association with polyribosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.