ArticleThe Plant cell1997
Assembly of Newly Imported Oxygen-Evolving Complex Subunits in Isolated Chloroplasts: Sites of Assembly and Mechanism of Binding.
Article in The Plant cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Arabidopsis conditional photosynthesis mutants abc1k1 and var2 accumulate partially processed thylakoid preproteins and are defective in chloroplast biogenesis.Communications biology · 2025Article
- The Role of Light-Dark Regulation of the Chloroplast ATP Synthase.Frontiers in plant science · 2017Article
- The N-terminal sequence of the extrinsic PsbP protein modulates the redox potential of Cyt b559 in photosystem II.Scientific reports · 2016Article
- Born in 1949 in postwar Japan.Photosynthesis research · 2016Article
- Quality Control of Photosystem II: The Mechanisms for Avoidance and Tolerance of Light and Heat Stresses are Closely Linked to Membrane Fluidity of the Thylakoids.Frontiers in plant science · 2016Review
- Ribosome nascent chain complexes of the chloroplast-encoded cytochrome b6 thylakoid membrane protein interact with cpSRP54 but not with cpSecY.Journal of bioenergetics and biomembranes · 2015Article
- MET1 is a thylakoid-associated TPR protein involved in photosystem II supercomplex formation and repair in Arabidopsis.The Plant cell · 2015Article
- Understanding the roles of the thylakoid lumen in photosynthesis regulation.Frontiers in plant science · 2013Review
- Intra-plastid protein trafficking: how plant cells adapted prokaryotic mechanisms to the eukaryotic condition.Biochimica et biophysica acta · 2013Review
- Operation of trans-thylakoid thiol-metabolizing pathways in photosynthesis.Frontiers in plant science · 2013Article
- Stoichiometry for binding and transport by the twin arginine translocation system.The Journal of cell biology · 2012Article
- The significance of protein maturation by plastidic type I signal peptidase 1 for thylakoid development in Arabidopsis chloroplasts.Plant physiology · 2010Article
- TLP18.3, a novel thylakoid lumen protein regulating photosystem II repair cycle.The Biochemical journal · 2007Article
- Article
- Amino acid sequences and solution structures of manganese stabilizing protein that affect reconstitution of Photosystem II activity.Photosynthesis research · 2003Article
- The chloroplast protein import channel Toc75: pore properties and interaction with transit peptides.Biophysical journal · 2002Article
- Component specificity for the thylakoidal Sec and Delta pH-dependent protein transport pathways.The Journal of cell biology · 1999Article
- Low density membranes are associated with RNA-binding proteins and thylakoids in the chloroplast of Chlamydomonas reinhardtii.The Journal of cell biology · 1998Article
- Expression, assembly and auxiliary functions of photosystem II oxygen-evolving proteins in higher plants.Photosynthesis researchReview
- Structure, function, and evolution of the PsbP protein family in higher plants.Photosynthesis researchReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined the assembly of the nuclear-encoded subunits of the oxygen-evolving complex (OEC) after their import into isolated intact chloroplasts. We showed that all three subunits examined (OE33, OE23, and OE17) partition between the thylakoid lumen and a site on the inner surface of the thylakoid membrane after import in a homologous system (e.g., pea or spinach subunits into pea or spinach chloroplasts, respectively). Although some interspecies protein import experiments resulted in OEC subunit binding, maize OE17 did not bind thylakoid membranes in chloroplasts isolated from peas. Newly imported OE33 and OE23 were washed from the membranes at the same concentrations of urea and NaCl as the native, indigenous proteins; this observation suggests that the former subunits are bound productively within the OEC. Inhibition of neither chloroplast protein synthesis nor light- or ATP-dependent energization of the thylakoid membrane significantly affected these assembly reactions, and we present evidence suggesting that incoming subunits actively displace those already bound to the thylakoid membrane. Transport of OE33 took place primarily in the stromal-exposed membranes and proceeded through a protease-sensitive, mature intermediate. Initial binding of OE33 to the thylakoid membrane occurred primarily in the stromal-exposed membranes, from where it migrated with measurable kinetics to the granal region. In contrast, OE23 assembly occurred in the granal membrane regions. This information is incorporated into a model of the stepwise assembly of oxygen-evolving photosystem II.
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