ArticleMolecular and cellular biochemistry2002
Effect of N1-guanyl-1,7-diaminoheptane, an inhibitor of deoxyhypusine synthase, on endothelial cell growth, differentiation and apoptosis.
Article in Molecular and cellular biochemistry, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Opposite effects of spermidine and GC7 in cell culture are dictated by distinct molecular targets.The Biochemical journal · 2025Article
- A genome-wide, CRISPR-based screen reveals new requirements for translation initiation and ubiquitination in driving adipogenic fate change.Genes & development · 2025Article
- Spermidine is a key polyamine required by intracellular parasites for survival within host erythrocytes.Science advances · 2025Article
- Spermidine mediates acetylhypusination of RIPK1 to suppress diabetes onset and progression.Nature cell biology · 2024Article
- Translation factor accelerating peptide bond formation on the ribosome: EF-P and eIF5A as entropic catalysts and a potential drug targets.BBA advances · 2023Article
- eIF5A-Independent Role of DHPS in p21International journal of molecular sciences · 2021Article
- Half Way to Hypusine-Structural Basis for Substrate Recognition by Human Deoxyhypusine Synthase.Biomolecules · 2020Article
- Insights Into the Binding Mechanism of GC7 to Deoxyhypusine Synthase inFrontiers in chemistry · 2020Article
- eIF5A is required for autophagy by mediating ATG3 translation.EMBO reports · 2018Article
- eIF5A2 is an alternative pathway for cell proliferation in cetuximab-treated epithelial hepatocellular carcinoma.American journal of translational research · 2016Article
- Hypusination of eukaryotic initiation factor 5A via cAMP-PKA-ERK1/2 pathway is required for ligand-induced downregulation of LH receptor mRNA expression in the ovary.Molecular and cellular endocrinology · 2015Article
- Deoxyhypusine synthase (DHPS) inhibitor GC7 induces p21/Rb-mediated inhibition of tumor cell growth and DHPS expression correlates with poor prognosis in neuroblastoma patients.Cellular oncology (Dordrecht, Netherlands) · 2014Article
- Cisplatin sensitivity is enhanced in non-small cell lung cancer cells by regulating epithelial-mesenchymal transition through inhibition of eukaryotic translation initiation factor 5A2.BMC pulmonary medicine · 2014Article
- Inhibition of eIF5A results in aberrant uterine natural killer cell function and embryo loss in mice.American journal of reproductive immunology (New York, N.Y. : 1989) · 2014Article
- Article
- Roles of eukaryotic initiation factor 5A2 in human cancer.International journal of biological sciences · 2013Review
- Implications of the Use of Eukaryotic Translation Initiation Factor 5A (eIF5A) for Prognosis and Treatment of Hepatocellular Carcinoma.International journal of hepatology · 2012Article
- Deoxyhypusine synthase haploinsufficiency attenuates acute cytokine signaling.Cell cycle (Georgetown, Tex.) · 2011Article
- The role of polyamines in supporting growth of mammalian cells is mediated through their requirement for translation initiation and elongation.The Journal of biological chemistry · 2010Article
- Inhibition of HIV-1 gene expression by Ciclopirox and Deferiprone, drugs that prevent hypusination of eukaryotic initiation factor 5A.Retrovirology · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An unusual amino acid, hypusine [Nepsilon-(4-amino-2-hydroxybutyl)lysine], is formed post-translationally in a single cellular protein, the eukaryotic translation initiation factor 5A (eIF5A) by deoxyhypusine synthase and deoxyhypusine hydroxylase. Although eIF5A and its hypusine modification are essential for eukaryotic cell viability, the true physiological function of eIF5A is yet unknown. We have examined the effects of N1-guanyl-1,7-diaminoheptane (GC7), a potent inhibitor of deoxyhypusine synthase, on endothelial cell proliferation, differentiation and apoptosis. Upon treatment of human umbilical vein endothelial cells (HUVEC) with GC7, dose-dependent inhibition of hypusine formation and cellular proliferation was observed. GC7 at 10 microM caused almost complete inhibition of cellular hypusine synthesis and led to cytostasis of HUVEC. Pretreatment of HUVEC with GC7 up to 50 microM for 4 days had little effect on the attachment and differentiation of these cells on Matri-gel and did not cause induction of apoptosis. Instead, the GC7 pretreatment (96 h at 5-50 microM) elicited protective effects against apoptotic death of HUVEC induced by serum starvation. These results suggest that eIF-5A may be involved in expression of proteins essential for apoptosis of endothelial cells as well as those for cellular proliferation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.