ArticleNucleic acids research2002
High throughput parallel synthesis of oligonucleotides with 1536 channel synthesizer.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Fabrication of microcolumn arrays for high-throughput oligonucleotide synthesis using 3D printing.Scientific reports · 2025Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.Computational and structural biotechnology journal · 2021Review
- Review
- Comparative secretome analysis of different smut fungi and identification of plant cell death-inducing secreted proteins from Tilletia horrida.BMC plant biology · 2019Article
- An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer.Scientific reports · 2019Article
- Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Enzymatic fabrication of high-density RNA arrays.Angewandte Chemie (International ed. in English) · 2014Article
- Next generation 1536-well oligonucleotide synthesizer with on-the-fly dispense.Journal of biotechnology · 2014Article
- Frontiers and approaches to chemical synthesis of oligodeoxyribonucleotides.Molecules (Basel, Switzerland) · 2013Review
- Gas-phase cleavage and dephosphorylation of universal linker-bound oligodeoxynucleotides.Nucleosides, nucleotides & nucleic acids · 2010Article
- Verifying expressed transcript variants by detecting and assembling stretches of consecutive exons.Nucleic acids research · 2010Article
- Design of microarray probes for virus identification and detection of emerging viruses at the genus level.BMC bioinformatics · 2006Article
- Tandem oligonucleotide synthesis using linker phosphoramidites.Nucleic acids research · 2005Article
- Optimization of probe length and the number of probes per gene for optimal microarray analysis of gene expression.Nucleic acids research · 2004Article
- Linker phosphoramidite reagents for the attachment of the first nucleoside to underivatized solid-phase supports.Nucleic acids research · 2004Article
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Authors and funding
5 authors.
Funding
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Abstract
A 1536 channel oligonucleotide synthesizer, the MultiSyn, was developed with the capability to simultaneously synthesize 1536 oligonucleotides of 20mer length in 10 h. The instrument was designed to synthesize different sequences of various lengths in micro-wells and has synthesized oligonucleotides as long as 119 nt with reasonably good yields using CPG beads of 1000 A pore size. The instrument consists of four 384 channel synthesis modules. Phosphoramidite chemistry was employed and step yields as high as 99.3% were achieved. The enhancement of oligonucleotide synthesis throughput is accomplished by increasing the spatial density of reaction wells. We have identified several parameters that are critical in achieving a good synthesis yield and negligible failure rate in small reaction wells. The coefficient of variation (CV) of product yields in 1536 reaction wells was 20%. The quality of the product was examined by capillary electrophoresis and mass spectrometry. The instrument has robustly synthesized oligonucleotides of various lengths for use as primers and probes for PCR amplifications, oligonucleotide microarrays and genotyping applications. This high throughput oligonucleotide synthesizer is a useful instrument for genomic applications, which require tens of thousands of probes or primers in a short time.
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