ArticleNucleic acids research2002
Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Differences in plasma proteomes for active tuberculosis, latent tuberculosis and non-tuberculosis mycobacterial lung disease patients with and without ESAT-6/CFP10 stimulation.Proteome science · 2020Article
- RNA Binding Proteins and Gene Expression Regulation inFrontiers in cellular and infection microbiology · 2020Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Gene Expression inmBio · 2017Article
- The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes.PLoS pathogens · 2017Article
- The messenger RNA decapping and recapping pathway in Trypanosoma.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The CAF1-NOT complex of trypanosomes.Frontiers in genetics · 2014Review
- An AU-rich instability element in the 3'UTR mediates an increase in mRNA stability in response to expression of a dhh1 ATPase mutant.Translation (Austin, Tex.) · 2014Article
- Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate.Journal of cell science · 2012Article
- PolyA-specific ribonuclease (PARN-1) function in stage-specific mRNA turnover in Trypanosoma brucei.Eukaryotic cell · 2011Article
- Rapid decay of unstable Leishmania mRNAs bearing a conserved retroposon signature 3'-UTR motif is initiated by a site-specific endonucleolytic cleavage without prior deadenylation.Nucleic acids research · 2010Article
- The RNA helicase DHH1 is central to the correct expression of many developmentally regulated mRNAs in trypanosomes.Journal of cell science · 2010Article
- The role of deadenylation in the degradation of unstable mRNAs in trypanosomes.Nucleic acids research · 2009Article
- Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteins.RNA (New York, N.Y.) · 2009Article
- Mutational analysis of a Dcp2-binding element reveals general enhancement of decapping by 5'-end stem-loop structures.Nucleic acids research · 2009Article
- Heat shock causes a decrease in polysomes and the appearance of stress granules in trypanosomes independently of eIF2(alpha) phosphorylation at Thr169.Journal of cell science · 2008Article
- Deadenylation-independent stage-specific mRNA degradation in Leishmania.Nucleic acids research · 2008Article
- Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.RNA (New York, N.Y.) · 2004Article
- Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p.RNA (New York, N.Y.) · 2004Article
- A role for the exosome in the in vivo degradation of unstable mRNAs.RNA (New York, N.Y.) · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
mRNA turnover is a regulated process that contributes to the steady state level of cytoplasmic mRNA. The amount of each mRNA determines, to a large extent, the amount of protein produced by that particular transcript. In trypanosomes, there is little transcriptional regulation; therefore, differential mRNA stability significantly contributes to mRNA levels in each stage of the parasite life cycle. To investigate the enzymatic activities that contribute to mRNA turnover, we developed a cell-free system for mRNA turnover using the trypanosome Leptomonas seymouri. We identified a decapping activity that removed m(7)GDP from mRNAs that contain an m(7)GpppN cap at their 5' end. In yeast, the release of m(7)GDP by the pyrophosphatase Dcp1p/Dcp2p is a rate-limiting step in mRNA turnover. A secondary enzymatic activity, similar to the human cap scavenger activity, was identified in the trypanosome extracts. Both the human and trypanosome scavenger activities generate m(7)GMP from short capped RNA and are inhibited by addition in trans of m(7)GpppG. A third enzymatic activity uncovered in the parasite extracts functioned as a 3' to 5' exonuclease. Importantly, this exonuclease activity was stimulated by an AU-rich element present in the RNA. In summary, the cell-free system has defined several RNA turnover steps that likely contribute to regulated mRNA decay in trypanosomes.
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