ArticleNucleic acids research2002
High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 36 citations in OpenAlex.
- Spliceosomal Prp8 intein at the crossroads of protein and RNA splicing.PLoS biology · 2019Article
- Cisplatin protects mice from challenge ofEmerging microbes & infections · 2019Article
- Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins.Nature methods · 2014Article
- 5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases.The Journal of biological chemistry · 2012Article
- Structural and mutational studies of a hyperthermophilic intein from DNA polymerase II of Pyrococcus abyssi.The Journal of biological chemistry · 2011Article
- Crystallization and preliminary X-ray diffraction analysis on the homing endonuclease I-Dmo-I in complex with its target DNA.Acta crystallographica. Section F, Structural biology and crystallization communications · 2007Article
- NMR structure of a KlbA intein precursor from Methanococcus jannaschii.Protein science : a publication of the Protein Society · 2007Article
- Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue.Journal of molecular biology · 2007Article
- The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage.Nucleic acids research · 2007Article
- Functional analysis of the split Synechocystis DnaE intein in plant tissues by biolistic particle bombardment.Transgenic research · 2006Article
- Evolution of the MAT locus and its Ho endonuclease in yeast species.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.Nucleic acids research · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The homing endonuclease PI-SceI from Saccharo myces cerevisiae consists of two domains. The protein splicing domain I catalyzes the excision of the mature endonuclease (intein) from a precursor protein and the religation of the flanking amino acid sequences (exteins) to a functional protein. Furthermore, domain I is involved in binding and recognition of the specific DNA substrate. Domain II of PI-SceI, the endonuclease domain, which is structurally homologous to other homing endonucleases from the LAGLIDADG family, harbors the endonucleolytic center of PI-SceI, which in vivo initiates the homing process by introducing a double-strand cut in the approximately 35 bp recognition sequence. At 1.35 A resolution, the crystal structure of PI-SceI domain I provides a detailed view of the part of the protein that is responsible for tight and specific DNA binding. A geometry-based docking of the 75 degrees bent recognition sequence to the full-length protein implies a conformational change or hinge movement of a subdomain of domain I, the tongs part, that is predicted to reach into the major groove near base pairs +16 to +18.
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