ArticleProceedings of the National Academy of Sciences of the United States of America2002
Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 28 citations in OpenAlex.
- Human TSC2-null fibroblast-like cells induce hair follicle neogenesis and hamartoma morphogenesis.Nature communications · 2011Article
- A gene therapy approach for long-term normalization of blood pressure in hypertensive mice by ANP-secreting human skin grafts.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Gene therapy approaches for stem cell protection.Gene therapy · 2008Review
- Gene therapy and wound healing.Clinics in dermatologyReview
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8 authors at 3 institutions in 1 country.
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Abstract
For skin gene therapy, achieving prolonged high-level gene expression in a significant percentage of keratinocytes (KC) is difficult because we cannot selectively target KC stem cells. We now demonstrate that topical colchicine treatment can be used to select, in vivo, KC progenitor cells transduced with the multidrug resistance gene (MDR1). When human skin equivalents containing MDR1-transduced KC were grafted onto immunocompromised mice, topical colchicine treatments significantly increased (7-fold) the percentage of KC expressing MDR1, compared to vehicle-treated controls, for up to 24 wk. Topical colchicine treatment also significantly enhanced the amount of MDR1 protein expressed in individual KC. Furthermore, quantitative real-time PCR analysis of MDR1 transgene copy number demonstrates that topical colchicine treatment selects and enriches for KC progenitor cells in the skin that contain and express MDR1. For clinical skin gene therapy applications, this in vivo selection approach promises to enhance both the duration and expression level of a desired therapeutic gene in KC, by linking its expression to the MDR1 selectable marker gene.
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