ArticleThe EMBO journal2002
RPD3 is required for the inactivation of yeast ribosomal DNA genes in stationary phase.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 133 citations in OpenAlex.
- Changes in macromolecular content and AOX activity of Ustilago maydis: from exponential to stationary phase.Archives of microbiology · 2025Article
- Single-cell imaging of protein dynamics of paralogs reveals sources of gene retention.iScience · 2025Article
- Cytoophidium complexes resonate with cell fates.Cellular and molecular life sciences : CMLS · 2025Article
- Psoralen Crosslinking-Chromatin Endogenous Cleavage Assay to Examine Histone DNA Interactions of Active and Inactive rRNA Genes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Establishment of closed 35S ribosomal RNA gene chromatin in stationary Saccharomyces cerevisiae cells.Nucleic acids research · 2024Article
- Reaching for the off switch in nucleolar dominance.The Plant journal : for cell and molecular biology · 2023Article
- Review
- Quiescence inAnnual review of genetics · 2022Review
- Facultative heterochromatin formation in rDNA is essential for cell survival during nutritional starvation.Nucleic acids research · 2022Article
- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Article
- Fast and inexpensive whole-genome sequencing library preparation from intact yeast cells.G3 (Bethesda, Md.) · 2021Article
- Is There a Histone Code for Cellular Quiescence?Frontiers in cell and developmental biology · 2021Review
- Nascent Transcript Folding Plays a Major Role in Determining RNA Polymerase Elongation Rates.Molecular cell · 2020Article
- Molecular Topology of RNA Polymerase I Upstream Activation Factor.Molecular and cellular biology · 2020Article
- Probing Mechanisms of Transcription Elongation Through Cell-to-Cell Variability of RNA Polymerase.Biophysical journal · 2020Article
- The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human.PLoS genetics · 2019Article
- Chromatin Remodeling Factors Isw2 and Ino80 Regulate Chromatin, Replication, and Copy Number of theGenetics · 2018Article
- Genomic Copy-Number Loss Is Rescued by Self-Limiting Production of DNA Circles.Molecular cell · 2018Article
- Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
rRNA transcription in Saccharomyces cerevisiae is performed by RNA polymerase I and regulated by changes in growth conditions. During log phase, approximately 50% of the ribosomal DNA (rDNA) genes in each cell are transcribed and maintained in an open, psoralen-accessible conformation. During stationary phase, the percentage of open rDNA genes is greatly reduced. In this study we found that the Rpd3 histone deacetylase was required to inactivate (close) individual rDNA genes as cells entered stationary phase. Even though approximately 50% of the rDNA genes remained open during stationary phase in rpd3Delta mutants, overall rRNA synthesis was still reduced. Using electron microscopy of Miller chromatin spreads, we found that the number of RNA polymerases transcribing each open gene in the rpd3Delta mutant was significantly reduced when cells grew past log phase. Bulk levels of histone H3 and H4 acetylation were reduced during stationary phase in an RPD3-dependent manner. However, histone H3 and H4 acetylation was not significantly altered at the rDNA locus in an rpd3Delta mutant. Rpd3 therefore regulates the number of open rDNA repeats.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.