ArticleProceedings of the National Academy of Sciences of the United States of America2002
Sequence-specific DNA binding by the vnd/NK-2 homeodomain of Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- NK2.2/Vnd acts as a transcriptional activator of deadpan to maintain neuroblast identity in Drosophila embryos.Genes & genomics · 2026Article
- Thyroid transcription factors in development, differentiation and disease.Nature reviews. Endocrinology · 2015Review
- Integrated cistromic and expression analysis of amplified NKX2-1 in lung adenocarcinoma identifies LMO3 as a functional transcriptional target.Genes & development · 2013Article
- Genome-wide screens for in vivo Tinman binding sites identify cardiac enhancers with diverse functional architectures.PLoS genetics · 2013Article
- The alternative protein isoform NK2B, encoded by the vnd/NK-2 proneural gene, directly activates transcription and is expressed following the start of cells differentiation.Nucleic acids research · 2011Article
- Novel computational analysis of protein binding array data identifies direct targets of Nkx2.2 in the pancreas.BMC bioinformatics · 2011Article
- The HMX/NKX homeodomain protein MLS-2 specifies the identity of the AWC sensory neuron type via regulation of the ceh-36 Otx gene in C. elegans.Development (Cambridge, England) · 2010Article
- Identification and analysis of vnd/NK-2 homeodomain binding sites in genomic DNA.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Mapping activation and repression domains of the vnd/NK-2 homeodomain protein.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Mutations that affect the ability of the vnd/NK-2 homeoprotein to regulate gene expression: transgenic alterations and tertiary structure.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The ventral nervous system defective (vnd)/NK-2 homeodomain and some flanking amino acid residues were expressed in Escherichia coli, purified to homogeneity, and the protein was covalently coupled to Sepharose. Oligodeoxynucleotides that contained 16-bp random sequences were purified by vnd/NK-2 affinity column chromatography, cloned, and sequenced. The consensus nucleotide sequence of the vnd/NK-2 homeodomain binding site was shown to be T(T/C)AAGTG(G/C). The apparent equilibrium dissociation constant (K(D)) of the vnd/NK-2 homeodomain for the consensus sequence is 1.9 x 10(-10) M. In addition, results of competition between oligodeoxynucleotides for binding to the vnd/NK-2 homeodomain and determination of the apparent K(D) values of oligodeoxynucleotides that differ from the consensus sequence by only a single base pair demonstrate that the four central nucleotides, AAGT, in this sequence play a major role in determining the affinity of binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.