ArticleProceedings of the National Academy of Sciences of the United States of America2002
Binding of the Escherichia coli response regulator CheY to its target measured in vivo by fluorescence resonance energy transfer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers.
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Who cites it
131 citing papers in PubMed.
- Correlation of CheY-P concentration and motor behavior during attractant adaptation in singleScience advances · 2026Article
- Toolbox of FRET-based c-di-GMP biosensors and its FRET-To-Sort application for genome-wide mapping of c-di-GMP regulation.Nature communications · 2026Article
- The dynamic response of the bacterial flagellar motor to its direct intracellular input signal.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Spontaneous switching in a protein signalling array reveals near-critical cooperativity.Nature physics · 2026Article
- Article
- Untangling the Complexity of Two-Component Signal Transduction in Bacteria.Microorganisms · 2025Review
- Pseudomonas aeruginosa Performs Chemotaxis to All Major Human Neurotransmitters.Microbial biotechnology · 2025Review
- Direct Quantification of Protein-Protein Interactions in Living Bacterial Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Slower swimming promotes chemotactic encounters between bacteria and small phytoplankton.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- MotY modulates proton-driven flagellar motor output in Pseudomonas aeruginosa.BMC microbiology · 2024Article
- Seeing with an extra sense.Current biology : CB · 2024Article
- Time-reversal symmetry breaking in the chemosensory array reveals a general mechanism for dissipation-enhanced cooperative sensing.Nature communications · 2024Article
- CheB localizes to polar receptor arrays during repellent adaptation.Science advances · 2024Article
- Article
- Flagellar dynamics reveal fluctuations and kinetic limit in the Escherichia coli chemotaxis network.Scientific reports · 2023Article
- Attractant and repellent induce opposing changes in the four-helix bundle ligand-binding domain of a bacterial chemoreceptor.PLoS biology · 2023Article
- A nonequilibrium allosteric model for receptor-kinase complexes: The role of energy dissipation in chemotaxis signaling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Trade-offs between cost and information in cellular prediction.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- The Chemoreceptor Sensory Adaptation System Produces Coordinated Reversals of the Flagellar Motors on an Escherichia coli Cell.Journal of bacteriology · 2022Article
71 more citing papers are in PubMed but not listed here.
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Escherichia coli chemotaxis, signaling depends on modulation of the level of phosphorylation of CheY, a small protein that couples receptors and flagellar motors. Working in vivo, we used fluorescence resonance energy transfer (FRET) to measure the interaction of CheY approximately P with its target, FliM. Binding of CheY approximately P to FliM was found to be much less cooperative than motor switching; however, under the conditions of our experiment, most of the FliM appeared to be in the cytoplasm. We studied signal processing times in the chemotaxis pathway by measuring the changes in CheY approximately P binding to FliM on flash release of caged chemoeffectors. Following sudden addition of attractant, the amount of CheY approximately P bound to FliM decayed exponentially with a rate constant of about 2 s(-1). Following sudden addition of repellent, FliM occupancy increased with a rate constant of about 20 s(-1). Using these data, we were able to construct a simple model for the chemotactic pathway and to estimate values of rate constants for several key reactions.
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