ArticlePlant physiology1993
DNA Strand-Transfer Activity in Pea (Pisum sativum L.) Chloroplasts.
Article in Plant physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- Chloroplastic RecA protein fromPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Intercompartmental Piecewise Gene Transfer.Genes · 2017Review
- Evolutionary origin of highly repetitive plastid genomes within the clover genus (Trifolium).BMC evolutionary biology · 2014Article
- A conserved lysine residue of plant Whirly proteins is necessary for higher order protein assembly and protection against DNA damage.Nucleic acids research · 2012Article
- Suppression of repeat-mediated gross mitochondrial genome rearrangements by RecA in the moss Physcomitrella patens.The Plant cell · 2009Article
- Characterization of replication origins flanking the 23S rRNA gene in tobacco chloroplast DNA.Plant molecular biology · 1996Article
- A gene encoding a truncated large subunit of Rubisco is transcribed and salt-inducible in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
- Radiation-sensitive mutants of Arabidopsis thaliana.Genetics · 1995Article
- Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA.Molecular and cellular biology · 1995Article
- Purification and characterization of a DNA strand transferase from broccoli.Plant physiology · 1995Article
- The search for the right partner: homologous pairing and DNA strand exchange proteins in eukaryotes.Experientia · 1994Review
- Treatment of pea (Pisum sativum L.) protoplasts with DNA-damaging agents induces a 39-kilodalton chloroplast protein immunologically related to Escherichia coli RecA.Plant physiology · 1993Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The occurrence of DNA recombination in plastids of higher plants is well documented. However, little is known at the enzymic level. To begin dissecting the biochemical mechanism(s) involved we focused on a key step: strand transfer between homologous parental DNAs. We detected a RecA-like strand transfer activity in stromal extracts from pea (Pisum sativum L.) chloroplasts. Formation of joint molecules requires Mg2+, ATP, and homologous substrates. This activity is inhibited by excess single-stranded DNA (ssDNA), suggesting a necessary stoichiometric relation between enzyme and ssDNA. In a novel assay with Triton X-100-permeabilized chloroplasts, we also detected strand invasion of the endogenous chloroplast DNA by 32P-labeled ssDNA complementary to the 16S rRNA gene. Joint molecules, analyzed by electron microscopy, contained the expected displacement loops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.