ArticlePlant physiology1995
[beta]-Tubulin Accumulation and DNA Replication in Imbibing Tomato Seeds.
Article in Plant physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Biopolymer-based seed biopriming with fungal endophytes suppresses Fusarium wilt and modulates growth in tomato.Frontiers in plant science · 2026Article
- Microtubule self-organisation during seed germination in Arabidopsis.BMC biology · 2020Article
- Feedback Microtubule Control and Microtubule-Actin Cross-talk inMolecular & cellular proteomics : MCP · 2017Article
- Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed.Plant biotechnology journal · 2012Article
- Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.Plant physiology · 2009Article
- ABA inhibits embryo cell expansion and early cell division events during coffee (Coffea arabica 'Rubi') seed germination.Annals of botany · 2008Article
- Transient occurrence of seed germination processes during coffee post-harvest treatment.Annals of botany · 2007Article
- Gene expression programs during Brassica oleracea seed maturation, osmopriming, and germination are indicators of progression of the germination process and the stress tolerance level.Plant physiology · 2005Article
- The role of the cell cycle machinery in resumption of postembryonic development.Plant physiology · 2005Article
- Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not.Plant physiology · 2000Article
- Characterization and expression of plasma and tonoplast membrane aquaporins in primed seed of Brassica napus during germination under stress conditions.Plant molecular biology · 1999Article
- Developmental expression and regulation by light of two closely related beta-tubulin genes in Lupinus albus.Plant molecular biology · 1996Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The activation of the cell cycle in embryo root tips of imbibing tomato (Lycopersicon esculentum Mill. cv Lerica) seeds was studied by flow cytometric analyses of the nuclear DNA content and by immunodelection of [beta]-tubulin. With dry seeds, flow cytometric profiles indicated that the majority of the cells were arrested at the G1 phase of the cell cycle. In addition, [beta]-tubulin was not detectable on western blots. Upon imbibition of water, the number of cells in G2 started to increase after 24 h, and a 55-kD [beta]-tubulin signal was detected between 24 and 48 h. Two-dimensional immunoblots revealed at least three different [beta]-tubulin isotypes. Thus, [beta]-tubulin accumulation and DNA replication were induced during osmotic priming. These processes, as well as seed germination rate, were enhanced upon subsequent imbibition of water, compared with control seeds that imbibed but were not primed. By contrast, when aged seeds imbibed, DNA replication, [beta]-tubulin accumulation, and germination were delayed. In all cases studied, both DNA replication and [beta]-tubulin accumulation preceded visible germination. We suggest that activation of these cell-cycle-related processes is a prerequisite for tomato seed germination. Furthermore, [beta]-tubulin expression can be used as a parameter for following the initial processes that are activated during seed imbibition.
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Registered trials
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