ArticlePlant physiology1995
RNA-Binding Characteristics of a Ribonucleoprotein from Spinach Chloroplast.
Article in Plant physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- The Chloroplast Ribonucleoprotein CP33B Quantitatively Binds thePlants (Basel, Switzerland) · 2020Article
- pTAC10, an S1-domain-containing component of the transcriptionally active chromosome complex, is essential for plastid gene expression in Arabidopsis thaliana and is phosphorylated by chloroplast-targeted casein kinase II.Photosynthesis research · 2018Article
- The effect of different 3' untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco.Plant molecular biology · 2011Article
- HCF152, an Arabidopsis RNA binding pentatricopeptide repeat protein involved in the processing of chloroplast psbB-psbT-psbH-petB-petD RNAs.The Plant cell · 2003Article
- The sequence and secondary structure of the 3'-UTR affect 3'-end maturation, RNA accumulation, and translation in tobacco chloroplasts.Plant molecular biology · 2000Article
- RNA-binding characteristics of the chloroplast S1-like ribosomal protein CS1.Nucleic acids research · 2000Article
- Altering the 3 UTR endonucleolytic cleavage site of a Chlamydomonas chloroplast mRNA affects 3-end maturation in vitro but not in vivo.Plant molecular biology · 1999Article
- 3'-Processed mRNA is preferentially translated in Chlamydomonas reinhardtii chloroplasts.Molecular and cellular biology · 1998Article
- Characterization of protein-binding to the spinach chloroplast psbA mRNA 5' untranslated region.Nucleic acids research · 1998Article
- The sequence and structure of the 3'-untranslated regions of chloroplast transcripts are important determinants of mRNA accumulation and stability.Plant molecular biology · 1998Article
- A Nuclear Mutation That Affects the 3[prime] Processing of Several mRNAs in Chlamydomonas Chloroplasts.The Plant cell · 1997Article
- Addition of destabilizing poly (A)-rich sequences to endonuclease cleavage sites during the degradation of chloroplast mRNA.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The 3' untranslated regions of chloroplast genes in Chlamydomonas reinhardtii do not serve as efficient transcriptional terminators.Molecular & general genetics : MGG · 1996Article
- Regulation of gene expression in chloroplasts of higher plants.Plant molecular biology · 1996Review
- CSP41, a sequence-specific chloroplast mRNA binding protein, is an endoribonuclease.The Plant cell · 1996Article
- Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins.The EMBO journal · 1996Article
- Phosphorylation of a chloroplast RNA-binding protein changes its affinity to RNA.Nucleic acids research · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A chloroplast (nuclear-encoded) RNA-binding protein (28RNP) was previously purified from spinach (Spinacia oleracea). This 28RNP was found to be the major RNA-binding protein co-purified during the isolation scheme of 3[prime] end RNA-processing activity of several chloroplastic genes. To learn more about the possible involvement of 28RNP in the 3[prime] end RNA-processing event, we investigated the RNA-binding properties and the location of the protein in the chloroplast. We found that recombinant Escherichia coliexpressed 28RNP binds with apparently the same affinity to every chloroplastic 3[prime] end RNA that was analyzed, as well as to RNAs derived from the 5[prime] end or the coding region of some chloroplastic genes. Differences in the RNA-binding affinities for some chloroplastic 3[prime] end RNAs were observed when the recombinant 28RNP was compared with the "native" 28RNP in the chloroplast-soluble protein extract. In addition, we found that the 28RNP is not associated with either thylakoid-bound or soluble polysomes in which a great portion of the chloroplast rRNA and mRNA are localized. These results suggest that the native 28RNP binds specifically to certain RNA molecules in the chloroplast in which other components (possibly proteins) and/or posttranslational modifications are involved in determining RNA-binding specificity of the 28RNP.
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Registered trials
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