ArticleInfection and immunity2002
Immortalization of human urethral epithelial cells: a model for the study of the pathogenesis of and the inflammatory cytokine response to Neisseria gonorrhoeae infection.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 63 citations in OpenAlex.
- NLRP3 Inflammasome in Gynecologic Inflammatory Diseases: Mechanisms, Pathophysiology, and Therapeutic Strategies.Journal of inflammation research · 2025Review
- Combined transgene immortalized urothelial cells capable of reprogramming and hepatic differentiation.Biochemistry and biophysics reports · 2022Article
- Tissue Models forFrontiers in cellular and infection microbiology · 2022Review
- Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- TheVaccines · 2020Article
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- Biomimetic Human Tissue Model for Long-Term Study ofFrontiers in microbiology · 2019Article
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- High content analysis in amyotrophic lateral sclerosis.Molecular and cellular neurosciences · 2017Review
- Is gonococcal disease preventable? The importance of understanding immunity and pathogenesis in vaccine development.Critical reviews in microbiology · 2016Review
- Inflammasomes and Their Role in Innate Immunity of Sexually Transmitted Infections.Frontiers in immunology · 2016Review
- Hexa-acylated lipid A is required for host inflammatory response to Neisseria gonorrhoeae in experimental gonorrhea.Infection and immunity · 2014Article
- Neisseria gonorrhoeae PIII has a role on NG1873 outer membrane localization and is involved in bacterial adhesion to human cervical and urethral epithelial cells.BMC microbiology · 2013Article
- Toll-like receptor 2-dependent activity of native major outer membrane protein proteosomes of Chlamydia trachomatis.Infection and immunity · 2013Article
- Modulation of HIV transmission by Neisseria gonorrhoeae: molecular and immunological aspects.Current HIV research · 2012Review
- Neisseria gonorrhoeae-mediated inhibition of apoptotic signalling in polymorphonuclear leukocytes.Infection and immunity · 2011Article
- Neisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells.Cellular microbiology · 2011Article
- Virulence determinants, drug resistance and mobile genetic elements of Laribacter hongkongensis: a genome-wide analysis.Cell & bioscience · 2011Article
- The Pathobiology of Neisseria gonorrhoeae Lower Female Genital Tract Infection.Frontiers in microbiology · 2011Article
- Factor H facilitates adherence of Neisseria gonorrhoeae to complement receptor 3 on eukaryotic cells.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The primary human urethral epithelial cells developed by our laboratory have been immortalized by transduction with a retroviral vector expressing the human papillomavirus E6E7 oncogenes. Analysis of telomerase expression and comparison to that in primary cells revealed detectable levels in the transduced human urethral epithelial cells. Immortalized urethral cells could be passaged over 20 times. Immunofluorescence microscopy studies showed that the immortalized cells were phenotypically similar and responded to gonococcal infection similarly to primary cells. Specifically, positive cytokeratin staining showed that the immortalized cells are keratinocytes; cell surface levels of human asialoglycoprotein receptor increase following gonococcal infection, and, like the primary cells, the immortalized urethral epithelial cells are CD14 negative. Using enzyme-linked immunosorbent assay, we found that interleukin-6 (IL-6) and IL-8 levels in primary urethral epithelial cell supernatants increase after challenge with N. gonorrhoeae. Likewise, the immortalized urethral epithelial cells produced higher levels of IL-6 and IL-8 cytokines in response to gonococcal infection. Cells challenged with a gonococcal lipid A msbB mutant produced reduced IL-6 and IL-8 levels when compared to the parent strain. Additionally, these data suggest that the 1291 msbB lipooligosaccharide may suppress cytokine induction.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.