ArticleInfection and immunity2002
Identification of a streptolysin S-associated gene cluster and its role in the pathogenesis of Streptococcus iniae disease.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 68 citations in OpenAlex.
- Review
- Streptococcal peptides and their roles in host-microbe interactions.Frontiers in cellular and infection microbiology · 2023Review
- Streptococcosis a Re-Emerging Disease in Aquaculture: Significance and Phytotherapy.Animals : an open access journal from MDPI · 2022Review
- Functional Analysis of Two NovelMicroorganisms · 2020Article
- Applications of Human Skin Microbiota in the Cutaneous Disorders for Ecology-Based Therapy.Frontiers in cellular and infection microbiology · 2020Review
- Propionibacterium acnes-induced immunopathology correlates with health and disease association.JCI insight · 2019Article
- β-HemolyticJournal of oral microbiology · 2019Article
- YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function.Chemical reviews · 2017Review
- Identification of the minimal cytolytic unit for streptolysin S and an expansion of the toxin family.BMC microbiology · 2015Article
- Streptolysin S of Streptococcus anginosus exhibits broad-range hemolytic activity.Medical microbiology and immunology · 2015Article
- Group B streptococcal haemolysin and pigment, a tale of twins.FEMS microbiology reviews · 2014Review
- Article
- Streptococcus iniae SF1: complete genome sequence, proteomic profile, and immunoprotective antigens.PloS one · 2014Article
- SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region.Acta veterinaria Scandinavica · 2013Article
- Propionibacterium acnes strain populations in the human skin microbiome associated with acne.The Journal of investigative dermatology · 2013Article
- Novel twin streptolysin S-like peptides encoded in the sag operon homologue of beta-hemolytic Streptococcus anginosus.Journal of bacteriology · 2013Article
- Innate immune response to Streptococcus iniae infection in zebrafish larvae.Infection and immunity · 2013Article
- Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis.BMC microbiology · 2012Article
- Streptolysin S-like virulence factors: the continuing sagA.Nature reviews. Microbiology · 2011Review
- Streptolysin S contributes to group A streptococcal translocation across an epithelial barrier.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus iniae causes meningoencephalitis and death in cultured fish species and soft-tissue infection in humans. We recently reported that S. iniae is responsible for local tissue necrosis and bacteremia in a murine subcutaneous infection model. The ability to cause bacteremia in this model is associated with a genetic profile unique to strains responsible for disease in fish and humans (J. D. Fuller, D. J. Bast, V. Nizet, D. E. Low, and J. C. S. de Azavedo, Infect. Immun. 69:1994-2000, 2001). S. iniae produces a cytolysin that confers a hemolytic phenotype on blood agar media. In this study, we characterized the genomic region responsible for S. iniae cytolysin production and assessed its contribution to virulence. Transposon (Tn917) mutant libraries of commensal and disease-associated S. iniae strains were generated and screened for loss of hemolytic activity. Analysis of two nonhemolytic mutants identified a chromosomal locus comprising 9 genes with 73% homology to the group A streptococcus (GAS) sag operon for streptolysin S (SLS) biosynthesis. Confirmation that the S. iniae cytolysin is a functional homologue of SLS was achieved by PCR ligation mutagenesis, complementation of an SLS-negative GAS mutant, and use of the SLS inhibitor trypan blue. SLS-negative sagB mutants were compared to their wild-type S. iniae parent strains in the murine model and in human whole-blood killing assays. These studies demonstrated that S. iniae SLS expression is required for local tissue necrosis but does not contribute to the establishment of bacteremia or to resistance to phagocytic clearance.
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