ArticleInfection and immunity2002
Secreted metalloprotease gene family of Microsporum canis.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 108 citations in OpenAlex.
- Remarkable Phenotypic Virulence Factors ofInternational journal of molecular sciences · 2024Pooled it
- Two-speed genome evolution drives pathogenicity in fungal pathogens of animals.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Mycoparasites, Gut Dwellers, and Saprotrophs: Phylogenomic Reconstructions and Comparative Analyses of Kickxellomycotina Fungi.Genome biology and evolution · 2023Article
- Dermatophyte infection: from fungal pathogenicity to host immune responses.Frontiers in immunology · 2023Review
- Comprehensive Assessment of the Virulence FactorsJournal of fungi (Basel, Switzerland) · 2021Article
- Gene expression profiling of protease and non-protease genes in Trichophyton mentagrophytes isolates from dermatophytosis patients by qRT-PCR analysis.Scientific reports · 2021Article
- Microbial enzymes catalyzing keratin degradation: Classification, structure, function.Biotechnology advances · 2020Review
- Azo dying of α-keratin material improves microbial keratinase screening and standardization.Microbial biotechnology · 2020Article
- Identification of keratinases from Fervidobacterium islandicum AW-1 using dynamic gene expression profiling.Microbial biotechnology · 2020Article
- Genes Encoding Proteolytic Enzymes Fungalysin and Subtilisin in Dermatophytes of Human and Animal Origin: A Comparative Study.Mycopathologia · 2020Article
- Article
- Fungal infections in animals: a patchwork of different situations.Medical mycology · 2018Review
- Selection and validation of reference genes for qRT-PCR analysis of gene expression in Microsporum canis growing under different adhesion-inducing conditions.Scientific reports · 2018Article
- Article
- A highly conserved metalloprotease effector enhances virulence in the maize anthracnose fungus Colletotrichum graminicola.Molecular plant pathology · 2016Article
- Transcription profile of Trichophyton rubrum conidia grown on keratin reveals the induction of an adhesin-like protein gene with a tandem repeat pattern.BMC genomics · 2016Article
- Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina.Applied microbiology and biotechnology · 2015Article
- Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Article
- Independent expansion of zincin metalloproteinases in Onygenales fungi may be associated with their pathogenicity.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-high percentage of identity with both A. fumigatus MEP and Aspergillus oryzae neutral protease I genes. At the amino acid level, they all contained an HEXXH consensus sequence, confirming that these M. canis genes (MEP genes) encode a zinc-containing metalloprotease gene family. Furthermore, MEP3 was found to be the gene encoding a previously isolated M. canis 43.5-kDa keratinolytic metalloprotease, and was successfully expressed as an active recombinant enzyme in Pichia pastoris. Reverse transcriptase nested PCR performed on total RNA extracted from the hair of M. canis-infected guinea pigs showed that at least MEP2 and MEP3 are produced during the infection process. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.