ArticleInfection and immunity2002
Characterization of P40, a cytadhesin of Mycoplasma agalactiae.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 68 citations in OpenAlex.
- Immune Responses Induced by Recombinant Membrane Proteins ofVaccines · 2025Article
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- A novel fusion protein candidate for the serodiagnosis of Mycoplasma agalactiae infection.BMC veterinary research · 2022Article
- Eating the Enemy: Mycoplasma Strategies to Evade Neutrophil Extracellular Traps (NETs) Promoting Bacterial Nucleotides Uptake and Inflammatory Damage.International journal of molecular sciences · 2022Review
- Host cell interactions of novel antigenic membrane proteins of Mycoplasma agalactiae.BMC microbiology · 2022Article
- Vaccines for Mycoplasma Diseases of Small Ruminants: A Neglected Area of Research.Pathogens (Basel, Switzerland) · 2022Review
- Review
- Molecular Identification of Mycoplasma agalactiae in Iran Based on P30 Gene.Archives of Razi Institute · 2021Article
- A novel chimeric recombinant protein PDHB-P80 ofMolecular biology research communications · 2020Article
- Genomic Islands in Mycoplasmas.Genes · 2020Review
- Article
- Novel Candidates for Vaccine Development AgainstVaccines · 2019Review
- Contagious Agalactia In Sheep And Goats: Current Perspectives.Veterinary medicine (Auckland, N.Z.) · 2019Review
- Identification of targets of monoclonal antibodies that inhibit adhesion and growth in Mycoplasma mycoides subspecies mycoides.Veterinary immunology and immunopathology · 2018Article
- Variable Membrane Protein A of Flavescence Dorée Phytoplasma Binds the Midgut Perimicrovillar Membrane of Euscelidius variegatus and Promotes Adhesion to Its Epithelial Cells.Applied and environmental microbiology · 2018Article
- Vpma phase variation is important for survival and persistence of Mycoplasma agalactiae in the immunocompetent host.PLoS pathogens · 2017Article
- Sheep primary cells as in vitro models to investigate Mycoplasma agalactiae host cell interactions.Pathogens and disease · 2015Article
- Heterologous expression and processing of the flavescence dorée phytoplasma variable membrane protein VmpA in Spiroplasma citri.BMC microbiology · 2015Article
- Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An immunodominant protein, P40, of Mycoplasma agalactiae was analyzed genetically and functionally. The gene encoding P40 was cloned from type strain PG2, sequenced, submitted to point mutagenesis in order to convert mycoplasma-specific TGA(Trp) codon to the universal TGG(Trp) codon, and subsequently expressed in Escherichia coli. Nucleotide sequence-derived amino acid sequence comparisons revealed a similarity of P40 to the adhesin P50 of Mycoplasma hominis and to protein P89 of Spiroplasma citri, which is expected to be involved in adhesion. The amino acid sequence of P40 revealed a recognition site for a signal peptidase and strong antigenic and hydrophilic motifs in the C-terminal domain. Triton X-114 phase partitioning confirmed that P40 is a membrane protein. Fab fragments of antibodies directed against recombinant purified P40 significantly inhibited adherence of M. agalactiae strains PG2 to lamb joint synovial cells LSM 192. Sera taken sequentially from sheep infected with PG2 revealed that P40 induced a strong and persistent immune response that gave strong signals on immunoblots containing recombinant P40 even 3 months after infection. The gene encoding P40 was present in a single copy in all of the 26 field strains of M. agalactiae analyzed and was not detected in closely related mycoplasma species. P40 was expressed as a protein with an apparent molecular mass of 37 kDa on sodium dodecyl sulfate-acrylamide gels by all M. agalactiae strains except for serotype C strains, which showed nonsense mutations in their p40 genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.