ArticleInfection and immunity2002
Identification and characterization of lpfABCC'DE, a fimbrial operon of enterohemorrhagic Escherichia coli O157:H7.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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93 citing papers in PubMed.
- Distribution of putative adhesins in Shiga toxin-producing Escherichia coli (STEC) strains isolated from different sources in Chile.Epidemiology and infection · 2007Trial
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- Comparison of hybrid-and mono-pathotype Escherichia coli isolates from South Korea based on whole genome analysis and cytotoxicity assay.Journal of biomedical science · 2026Article
- Creating and leveraging bespoke large-scale knowledge graphs for comparative genomics and multi-omics drug discovery with SocialGene.bioRxiv : the preprint server for biology · 2024Article
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- Bacteria from gut microbiota associated with diarrheal infections in children promote virulence of Shiga toxin-producing and enteroaggregativeFrontiers in cellular and infection microbiology · 2022Article
- Extended-Spectrum β-Lactamase-Producing andApplied and environmental microbiology · 2021Article
- PchE Regulation ofInternational journal of molecular sciences · 2020Article
- Recent Advances in Our Understanding of the Diversity and Roles of Chaperone-Usher Fimbriae in FacilitatingFrontiers in cellular and infection microbiology · 2020Review
- Mechanisms ofFrontiers in microbiology · 2020Article
- Stress Resistance Development and Genome-Wide Transcriptional Response of Escherichia coli O157:H7 Adapted to Sublethal Thymol, Carvacrol, andApplied and environmental microbiology · 2018Article
- After the Fact(or): Posttranscriptional Gene Regulation in Enterohemorrhagic Escherichia coli O157:H7.Journal of bacteriology · 2018Review
- Pili Assembled by the Chaperone/Usher Pathway inEcoSal Plus · 2018Review
- Distribution of Major Pilin Subunit Genes Among Atypical EnteropathogenicFrontiers in microbiology · 2018Article
- Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:HApplied and environmental microbiology · 2017Article
- Functional expression of the entire adhesiome of Salmonella enterica serotype Typhimurium.Scientific reports · 2017Article
- Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer's patches interactions.Scientific reports · 2017Article
- Finding Regulators Associated with the Expression of the Long Polar Fimbriae in Enteropathogenic Escherichia coli.Journal of bacteriology · 2015Article
- Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes.PloS one · 2015Article
33 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
The mechanisms underlying the adherence of Escherichia coli O157:H7 and other enterohemorrhagic E. coli (EHEC) strains to intestinal epithelial cells are poorly understood. We have identified a chromosomal region (designated lpfABCC'DE) in EHEC O157:H7 containing six putative open reading frames that was found to be closely related to the long polar (LP) fimbria operon (lpf) of Salmonella enterica serovar Typhimurium, both in gene order and in conservation of the deduced amino acid sequences. We show that lpfABCC'DE is organized as an operon and that its expression is induced during the exponential growth phase. The lpf genes from EHEC strain EDL933 were introduced into a nonfimbriated (Fim(-)) E. coli K-12 strain, and the transformed strain produced fimbriae as visualized by electron microscopy and adhered to tissue culture cells. Anti-LpfA antiserum recognized a ca. 16-kDa LpfA protein when expressed under regulation of the T7 promoter system. The antiserum also cross-reacted with the LP fimbriae in immunogold electron microscopy and Western blot experiments. Isogenic E. coli O157:H7 lpf mutants derived from strains 86-24 and AGT300 showed slight reductions in adherence to tissue culture cells and formed fewer microcolonies compared with their wild-type parent strains. The adherence and microcolony formation phenotypes were restored when the lpf operon was introduced on a plasmid. We propose that LP fimbriae participate in the interaction of E. coli O157:H7 with eukaryotic cells by assisting in microcolony formation.
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