ArticlePlant physiology2002
Cloning and functional characterization of a beta-pinene synthase from Artemisia annua that shows a circadian pattern of expression.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Metabolomic and Single-Cell Transcriptomic Analyses Shed Light on Secondary Metabolite Profiling and Potential Developmental Dynamics of Glandular Trichomes in Artemisia argyi.Plant biotechnology journal · 2026Article
- Inhibitor treatment and subcellular localization analysis reveal the contribution of a cytosolic terpene synthase to the substantial release of anti-insect monoterpenes by Sphagneticola trilobata (L.) Pruski.Journal of advanced research · 2025Article
- Single-nucleus transcriptomics reveal the morphogenesis and artemisinin biosynthesis in Artemisia annua glandular trichomes.Nature communications · 2025Article
- Harnessing controlled-environment systems for enhanced production of medicinal plants.Journal of experimental botany · 2025Review
- Integrated multi-omics profiling reveals a landscape of dramatic metabolic defect inHorticulture research · 2024Article
- Article
- Unlocking Nature's Rhythms: Insights into Secondary Metabolite Modulation by the Circadian Clock.International journal of molecular sciences · 2024Review
- Terpene synthases in cucumber (Cucumis sativus) and their contribution to herbivore-induced volatile terpenoid emission.The New phytologist · 2022Article
- Article
- Light dominates the diurnal emissions of herbivore-induced volatiles in wild tobacco.BMC plant biology · 2021Article
- Two Origins, Two Functions: The Discovery of Distinct Secretory Ducts Formed during the Primary and Secondary Growth inPlants (Basel, Switzerland) · 2021Article
- Zingerone in the Flower ofMolecules (Basel, Switzerland) · 2020Article
- Article
- Regulation of the Rhythmic Emission of Plant Volatiles by the Circadian Clock.International journal of molecular sciences · 2017Review
- Volatile terpenoids: multiple functions, biosynthesis, modulation and manipulation by genetic engineering.Planta · 2017Review
- Structured plant metabolomics for the simultaneous exploration of multiple factors.Scientific reports · 2016Article
- Rapid Discovery and Functional Characterization of Terpene Synthases from Four Endophytic Xylariaceae.PloS one · 2016Article
- Isolation and Characterization of Three New Monoterpene Synthases from Artemisia annua.Frontiers in plant science · 2016Article
- The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase.Journal of experimental botany · 2015Article
- Plant terpenes: defense responses, phylogenetic analysis, regulation and clinical applications.3 Biotech · 2015Article
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Authors and funding
6 authors.
Funding
Abstract
Artemisia annua plants produce a broad range of volatile compounds, including monoterpenes, which contribute to the characteristic fragrance of this medicinal species. A cDNA clone, QH6, contained an open reading frame encoding a 582-amino acid protein that showed high sequence identity to plant monoterpene synthases. The prokaryotically expressed QH6 fusion protein converted geranyl diphosphate to (-)-beta-pinene and (-)-alpha-pinene in a 94:6 ratio. QH6 was predominantly expressed in juvenile leaves 2 weeks postsprouting. QH6 transcript levels were transiently reduced following mechanical wounding or fungal elicitor treatment, suggesting that this gene is not directly involved in defense reaction induced by either of these treatments. Under a photoperiod of 12 h/12 h (light/dark), the abundance of QH6 transcripts fluctuated in a diurnal pattern that ebbed around 3 h before daybreak (9th h in the dark phase) and peaked after 9 h in light (9th h in the light phase). The contents of (-)-beta-pinene in juvenile leaves and in emitted volatiles also varied in a diurnal rhythm, correlating strongly with mRNA accumulation. When A. annua was entrained by constant light or constant dark conditions, QH6 transcript accumulation continued to fluctuate with circadian rhythms. Under constant light, advanced cycles of fluctuation of QH6 transcript levels were observed, and under constant dark, the cycle was delayed. However, the original diurnal pattern could be regained when the plants were returned to the normal light/dark (12 h/12 h) photoperiod. This is the first report that monoterpene biosynthesis is transcriptionally regulated in a circadian pattern.
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