ArticlePlant physiology2002
Arabinoxylan biosynthesis in wheat. Characterization of arabinosyltransferase activity in Golgi membranes.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 68 citations in OpenAlex.
- Pathway-specific enzymes from bamboo and crop leaves biosynthesize anti-nociceptive C-glycosylated flavones.Communications biology · 2020Article
- A Bifunctional UDP-Sugar 4-Epimerase Supports Biosynthesis of Multiple Cell Surface Polysaccharides in Sinorhizobium meliloti.Journal of bacteriology · 2019Article
- Identification and characterization of some putative genes involved in arabinoxylan biosynthesis in3 Biotech · 2018Article
- A cytochrome P450 CYP71 enzyme expressed in Sorghum bicolor root hair cells participates in the biosynthesis of the benzoquinone allelochemical sorgoleone.The New phytologist · 2018Article
- The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2.Plant physiology · 2016Article
- Asparagus IRX9, IRX10, and IRX14A Are Components of an Active Xylan Backbone Synthase Complex that Forms in the Golgi Apparatus.Plant physiology · 2016Article
- Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Frontiers in plant science · 2016Article
- Grass Cell Walls: A Story of Cross-Linking.Frontiers in plant science · 2016Article
- Article
- XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The interconversion of UDP-arabinopyranose and UDP-arabinofuranose is indispensable for plant development in Arabidopsis.The Plant cell · 2011Article
- Update on mechanisms of plant cell wall biosynthesis: how plants make cellulose and other (1->4)-β-D-glycans.Plant physiology · 2011Review
- Biosynthesis of UDP-xylose and UDP-arabinose in Sinorhizobium meliloti 1021: first characterization of a bacterial UDP-xylose synthase, and UDP-xylose 4-epimerase.Microbiology (Reading, England) · 2011Article
- A glucurono(arabino)xylan synthase complex from wheat contains members of the GT43, GT47, and GT75 families and functions cooperatively.Plant physiology · 2010Article
- Xylan biosynthesis: news from the grass.Plant physiology · 2010Article
- The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone.Plant physiology · 2010Article
- Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis.The Plant cell · 2008Article
- Arabidopsis cytochrome P450 monooxygenase 71A13 catalyzes the conversion of indole-3-acetaldoxime in camalexin synthesis.The Plant cell · 2007Article
- The Arabidopsis irregular xylem8 mutant is deficient in glucuronoxylan and homogalacturonan, which are essential for secondary cell wall integrity.The Plant cell · 2007Article
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3 authors.
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Abstract
Arabinoxylan arabinosyltransferase (AX-AraT) activity was investigated using microsomes and Golgi vesicles isolated from wheat (Triticum aestivum) seedlings. Incubation of microsomes with UDP-[(14)C]-beta-L-arabinopyranose resulted in incorporation of radioactivity into two different products, although most of the radioactivity was present in xylose (Xyl), indicating a high degree of UDP-arabinose (Ara) epimerization. In isolated Golgi vesicles, the epimerization was negligible, and incubation with UDP-[(14)C]Ara resulted in formation of a product that could be solubilized with proteinase K. In contrast, when Golgi vesicles were incubated with UDP-[(14)C]Ara in the presence of unlabeled UDP-Xyl, the product obtained could be solubilized with xylanase, whereas proteinase K had no effect. Thus, the AX-AraT is dependent on the synthesis of unsubstituted xylan acting as acceptor. Further analysis of the radiolabeled product formed in the presence of unlabeled UDP-Xyl revealed that it had an apparent molecular mass of approximately 500 kD. Furthermore, the total incorporation of [(14)C]Ara was dependent on the time of incubation and the amount of Golgi protein used. AX-AraT activity had a pH optimum at 6, and required the presence of divalent cations, Mn(2+) being the most efficient. In the absence of UDP-Xyl, a single arabinosylated protein with an apparent molecular mass of 40 kD was radiolabeled. The [(14)C]Ara labeling became reversible by adding unlabeled UDP-Xyl to the reaction medium. The possible role of this protein in arabinoxylan biosynthesis is discussed.
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