ArticlePlant physiology2002
F-actin-dependent endocytosis of cell wall pectins in meristematic root cells. Insights from brefeldin A-induced compartments.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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Who cites it
109 citing papers in PubMed, 264 citations in OpenAlex.
- Memory in the wall: expanding our understanding of the roles of plant cell walls.The New phytologist · 2026Review
- Storming the barricades of rhamnogalacturonan-II synthesis and function.The Plant cell · 2025Review
- Mapping the membrane orientation of auxin homeostasis regulators PIN5 and PIN8 in Arabidopsis thaliana root cells reveals their divergent topology.Plant methods · 2024Article
- Differences in the Occurrence of Cell Wall Components between Distinct Cell Types in Glands ofInternational journal of molecular sciences · 2023Article
- Genome-Wide Identification and Expression Analysis of ACTIN Family Genes in the Sweet Potato and Its Two Diploid Relatives.International journal of molecular sciences · 2023Article
- The Impact of Gold Nanoparticles on Somatic Embryogenesis Using the Example ofInternational journal of molecular sciences · 2023Article
- Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth ofPlants (Basel, Switzerland) · 2022Article
- O-glycosylation of the extracellular domain of pollen class I formins modulates their plasma membrane mobility.Journal of experimental botany · 2022Article
- Dynamics of cell wall polysaccharides during the elongation growth of rye primary roots.Planta · 2022Article
- Spatial and temporal localization of SPIRRIG and WAVE/SCAR reveal roles for these proteins in actin-mediated root hair development.The Plant cell · 2021Article
- Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth.The protein journal · 2021Article
- From monocots to dicots: the multifold aspect of cell wall expansion.Journal of experimental botany · 2021Article
- Global Identification ofFrontiers in plant science · 2021Article
- Bundling up the Role of the Actin Cytoskeleton in Primary Root Growth.Frontiers in plant science · 2021Review
- Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress.Frontiers in cell and developmental biology · 2021Article
- Sec71 separates Golgi stacks inJournal of cell science · 2020Article
- Quantitative Structural Organization of Bulk Apical Membrane Traffic in Pollen Tubes.Plant physiology · 2020Article
- An Intrinsically Disordered Protein Interacts with the Cytoskeleton for Adaptive Root Growth under Stress.Plant physiology · 2020Article
- TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network.Nature communications · 2018Article
- Anaesthetics stop diverse plant organ movements, affect endocytic vesicle recycling and ROS homeostasis, and block action potentials in Venus flytraps.Annals of botany · 2018Article
49 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries. In plants, formation of large intracellular compartments in response to BFA treatment is a unique feature of some, but not all, cells. Here, we have analyzed assembly and distribution of BFA compartments in development- and tissue-specific contexts of growing maize (Zea mays) root apices. Surprisingly, these unique compartments formed only in meristematic cells of the root body. On the other hand, BFA compartments were absent from secretory cells of root cap periphery, metaxylem cells, and most elongating cells, all of which are active in exocytosis. We report that cell wall pectin epitopes counting rhamnogalacturonan II dimers cross-linked by borate diol diester, partially esterified (up to 40%) homogalacturonan pectins, and (1-->4)-beta-D-galactan side chains of rhamnogalacturonan I were internalized into BFA compartments. In contrast, Golgi-derived secretory (esterified up to 80%) homogalacturonan pectins localized to the cytoplasm in control cells and did not accumulate within characteristic BFA compartments. Latrunculin B-mediated depolymerization of F-actin inhibited internalization and accumulation of cell wall pectins within intracellular BFA compartments. Importantly, cold treatment and protoplasting prevented internalization of wall pectins into root cells upon BFA treatment. These observations suggest that cell wall pectins of meristematic maize root cells undergo rapid endocytosis in an F-actin-dependent manner.
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