ArticlePlant physiology2002
Isolation and characterization of a novel ribosome-inactivating protein from root cultures of pokeweed and its mechanism of secretion from roots.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 71 citations in OpenAlex.
- Cytotoxicity Effect of Quinoin, Type 1 Ribosome-Inactivating Protein from Quinoa Seeds, on Glioblastoma Cells.Toxins · 2021Article
- Article
- Purification of a ribosome-inactivating protein with antioxidation and root developer potencies fromPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Toxic proteins in plants.Phytochemistry · 2015Review
- Ribosome-inactivating and related proteins.Toxins · 2015Review
- Pokeweed antiviral protein, a ribosome inactivating protein: activity, inhibition and prospects.Toxins · 2015Review
- Immunotoxins constructed with ribosome-inactivating proteins and their enhancers: a lethal cocktail with tumor specific efficacy.Current pharmaceutical design · 2014Review
- Analysis of castor bean ribosome-inactivating proteins and their gene expression during seed development.Genetics and molecular biology · 2013Article
- Targeting an antimicrobial effector function in insect immunity as a pest control strategy.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Extracellular proteins in pea root tip and border cell exudates.Plant physiology · 2007Article
- Strategies for the engineered phytoremediation of toxic element pollution: mercury and arsenic.Journal of industrial microbiology & biotechnology · 2005Review
- Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes.Planta · 2005Article
- Root border-like cells of Arabidopsis. Microscopical characterization and role in the interaction with rhizobacteria.Plant physiology · 2005Article
- Proton-transfer-reaction mass spectrometry as a new tool for real time analysis of root-secreted volatile organic compounds in Arabidopsis.Plant physiology · 2004Article
- Isolation and characterization of an RIP (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity.Plant physiology · 2004Article
- Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production.Plant physiology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribosome-inactivating proteins are N-glycosidases that remove a specific adenine from the sarcin/ricin loop of the large rRNA, thus arresting protein synthesis at the translocation step. In the present study, a novel type I ribosome-inactivating protein, termed PAP-H, was purified from Agrobacterium rhizogenes-transformed hairy roots of pokeweed (Phytolacca americana). The protein was purified by anion- and cation-exchange chromatography. PAP-H has a molecular mass of 29.5 kD as detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its isoelectric point was determined to be 7.8. Yeast (Saccharomyces cerevisiae) ribosomes incubated with PAP-H released the 360-nucleotide diagnostic fragment from the 26S rRNA upon aniline treatment, an indication of its ribosome-inactivating activity. Using immunofluorescence microscopy, PAP-H was found to be located in the cell walls of hairy roots and root border cells. PAP-H was determined to be constitutively secreted as part of the root exudates, with its secretion enhanced by a mechanism mediated by ethylene induction. Purified PAP-H did not show in vitro antifungal activity against soil-borne fungi. In contrast, root exudates containing PAP-H as well as additional chitinase, beta-1,3-glucanase, and protease activities did inhibit the growth of soil-borne fungi. We found that PAP-H depurinates fungal ribosomes in vitro and in vivo, suggesting an additive mechanism that enables PAP-H to penetrate fungal cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.