ArticlePlant physiology2002
An endoplasmic reticulum-bound Ca(2+)/Mn(2+) pump, ECA1, supports plant growth and confers tolerance to Mn(2+) stress.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 184 citations in OpenAlex.
- Comparative Transcriptomic Analysis Reveals Conserved and Nutrient-Specific Responses to Nutrient Deficiencies in Quinoa.Plants (Basel, Switzerland) · 2026Article
- Genome-Wide Identification of the P-Type CaInternational journal of molecular sciences · 2026Article
- Chromosome-level genome assembly and population genomics analysis ofPlant diversity · 2026Article
- Genome-Wide Association Studies for Grain Micronutrient Concentration in Spring Wheat (Plants (Basel, Switzerland) · 2025Article
- An intracellular CPK-ECA1 phosphoregulatory circuit couples calcium signatures to ABA homeostasis for plant osmosensivity.Science advances · 2025Article
- Transcriptome-based analysis reveals key molecular mechanisms and functional characterization of MaCAX3 gene involved in manganese stress responses in mulberry plants.BMC plant biology · 2025Article
- Ion toxicity in waterlogged soils: mechanisms of root response and adaptive strategies.Frontiers in plant science · 2025Review
- Genome-Wide Identification and Expression Analysis ofInternational journal of molecular sciences · 2024Article
- Review
- Transcriptome Sequencing Analysis of Root in Soybean Responding to Mn Poisoning.International journal of molecular sciences · 2023Article
- Review
- Comparative Transcriptome Analysis Reveals Complex Physiological Response and Gene Regulation in Peanut Roots and Leaves under Manganese Toxicity Stress.International journal of molecular sciences · 2023Article
- The trans-Golgi-localized protein BICAT3 regulates manganese allocation and matrix polysaccharide biosynthesis.Plant physiology · 2022Article
- Genome-Wide Identification and Characterization of Banana CaInternational journal of molecular sciences · 2022Article
- CaProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Hydrogen Sulfide Alleviates Manganese Stress inInternational journal of molecular sciences · 2022Article
- Evolutionary and Regulatory Pattern Analysis of Soybean CaFrontiers in plant science · 2022Article
- Calcium channels and transporters: Roles in response to biotic and abiotic stresses.Frontiers in plant science · 2022Review
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- Manganese distribution in the Mn-hyperaccumulator Grevillea meisneri from New Caledonia.Scientific reports · 2021Article
15 more citing papers are in PubMed but not listed here.
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7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants can grow in soils containing highly variable amounts of mineral nutrients, like Ca(2+) and Mn(2+), though the mechanisms of adaptation are poorly understood. Here, we report the first genetic study to determine in vivo functions of a Ca(2+) pump in plants. Homozygous mutants of Arabidopsis harboring a T-DNA disruption in ECA1 showed a 4-fold reduction in endoplasmic reticulum-type calcium pump activity. Surprisingly, the phenotype of mutant plants was indistinguishable from wild type when grown on standard nutrient medium containing 1.5 mM Ca(2+) and 50 microM Mn(2+). However, mutants grew poorly on medium with low Ca(2+) (0.2 mM) or high Mn(2+) (0.5 mM). On high Mn(2+), the mutants failed to elongate their root hairs, suggesting impairment in tip growth processes. Expression of the wild-type gene (CAMV35S::ECA1) reversed these conditional phenotypes. The activity of ECA1 was examined by expression in a yeast (Saccharomyces cerevisiae) mutant, K616, which harbors a deletion of its endogenous calcium pumps. In vitro assays demonstrated that Ca(2+), Mn(2+), and Zn(2+) stimulated formation of a phosphoenzyme intermediate, consistent with the translocation of these ions by the pump. ECA1 provided increased tolerance of yeast mutant to toxic levels of Mn(2+) (1 mM) and Zn(2+)(3 mM), consistent with removal of these ions from the cytoplasm. These results show that despite the potential redundancy of multiple Ca(2+) pumps and Ca(2+)/H(+) antiporters in Arabidopsis, pumping of Ca(2+) and Mn(2+) by ECA1 into the endoplasmic reticulum is required to support plant growth under conditions of Ca(2+) deficiency or Mn(2+) toxicity.
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