ArticlePlant physiology2002
Molecular identification of cytosolic, patatin-related phospholipases A from Arabidopsis with potential functions in plant signal transduction.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 143 citations in OpenAlex.
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- Genome-wide screening and characterization of phospholipase A (PLA)-like genes in sorghum (Sorghum bicolor L.).Planta · 2024Article
- Comprehensive identification and functional characterization of GhpPLA gene family in reproductive organ development.BMC plant biology · 2023Article
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- Analysis of plant expression profiles revealed that aphid attack triggered dynamic defense responses in sorghum plant.Frontiers in genetics · 2023Article
- The functions of phospholipases and their hydrolysis products in plant growth, development and stress responses.Progress in lipid research · 2022Review
- The Reduced Longitudinal Growth Induced by Overexpression ofPlants (Basel, Switzerland) · 2021Article
- Genetic regulators of leaf size in Brassica crops.Horticulture research · 2021Review
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- GhPLP2 Positively Regulates Cotton Resistance to Verticillium Wilt by Modulating Fatty Acid Accumulation and Jasmonic Acid Signaling Pathway.Frontiers in plant science · 2021Article
- Phospholipase pPLAIIIα Increases Germination Rate and Resistance to Turnip Crinkle Virus when Overexpressed.Plant physiology · 2020Article
- Lipid Metabolism in Plants.Plants (Basel, Switzerland) · 2020Article
- Patatin-Related PhospholipasePlants (Basel, Switzerland) · 2020Article
- Overexpression of ginseng patatin-related phospholipaseJournal of ginseng research · 2020Article
- Comparative Transcriptome Analysis of Two Cucumber Cultivars with Different Sensitivity to Cucumber Mosaic Virus Infection.Pathogens (Basel, Switzerland) · 2020Article
- PuHSFA4a Enhances Tolerance To Excess Zinc by Regulating Reactive Oxygen Species Production and Root Development inPlant physiology · 2019Article
- The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola.Scientific reports · 2018Article
- Article
- Global gene expression in two potato cultivars in response to 'Candidatus Liberibacter solanacearum' infection.BMC genomics · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid activation of phospholipase A (PLA) by auxin or plant-pathogen interaction suggests a function in signal transduction for this enzyme, but the molecular identification of a cytosolic PLA carrying out this function remains open. We isolated four cDNA sequences from Arabidopsis (ecotype Columbia), AtPLA I, AtPLA IIA, AtPLA IVA, and AtPLA IVC, which are members of the patatin-related PLA gene family in plants and which are homologous to the animal Ca(2+)-independent PLA(2) gene family. Expression was measured by reverse transcriptase-polymerase chain reaction, and AtPLA I transcripts were found preferentially in shoots, AtPLA IIA and AtPLA IVA in roots, and AtPLA IVC in flowers. Transient expression of the four PLA-green fluorescent protein fusion proteins in tobacco (Nicotiana tabacum) leaves showed they were located in the cytosol and not in the vacuoles. Surprisingly, AtPLA::green fluorescent protein was also localized to chloroplasts. The enzymatic activity of the purified recombinant AtPLA IVA toward phosphatidylcholine was dependent on Ca(2+), saturated at 0.5 mM, and had a pH optimum of about 7.0. It had both PLA(1) and PLA(2) specificity. The enzyme showed in vitro highest sensitivity toward the PLA(2) inhibitors palmitoyltrifluoromethyl ketone (PACOCF(3), K(i) approximately 30 nM), arachidonyltrifluoromethyl ketone (AACOCF(3), K(i) approximately 25 microM), and tetrahydro-3-(1-naphtalenyl)-2H-pyran-2-one (K(i) approximately 200 nM) and was also sensitive to other previously used inhibitors 5,8,11,14-eicosatetraynoic acid (K(i) approximately 3 microM) and nordihydroguajaretic acid (K(i) approximately 15 microM). The influence of these PLA(2) inhibitors on elongation in etiolated Arabidopsis seedlings was tested, and tetrahydro-3-(1-naphtalenyl)-2H-pyran-2-one and 5,8,11,14-eicosatetraynoic acid inhibited hypocotyl elongation maximally at concentrations close to their K(i) in vitro.
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