ArticlePlant physiology1996
Changes in Cytokinin Content and Cytokinin Oxidase Activity in Response to Derepression of ipt Gene Transcription in Transgenic Tobacco Calli and Plants.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 114 citations in OpenAlex.
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- Profiles of Cytokinins Metabolic Genes and Endogenous Cytokinins Dynamics during Shoot Multiplication In Vitro ofInternational journal of molecular sciences · 2022Article
- Identification and Characterization of Key Genes Responsible for Weedy and Cultivar Growth Types in Soybean.Frontiers in genetics · 2022Article
- Plant Growth Regulators INCYDE and TD-K Underperform in Cereal Field Trials.Plants (Basel, Switzerland) · 2021Article
- Biochemical and molecular characterisation of exogenous cytokinin application on grain filling in rice.BMC plant biology · 2018Article
- Article
- Cell-Type-Specific Cytokinin Distribution within the Arabidopsis Primary Root Apex.The Plant cell · 2015Article
- Cytokinin levels and signaling respond to wounding and the perception of herbivore elicitors in Nicotiana attenuata.Journal of integrative plant biology · 2015Article
- Differential regulation of cytokinin oxidase genes and cytokinin-induced auxin biosynthesis by cellular cytokinin level in transgenic poplars.Plant cell reports · 2014Article
- Cytokinins.The arabidopsis book · 2014Article
- Development of single-vector Tet-on inducible systems with high sensitivity to doxycycline.Molecular biotechnology · 2012Article
- Consequences of transforming narrow leafed lupin (Lupinus angustifolius [L.]) with an ipt gene under control of a flower-specific promoter.Transgenic research · 2011Article
- The cytokinin receptors of Arabidopsis are located mainly to the endoplasmic reticulum.Plant physiology · 2011Article
- Photosynthesis, water use, and root viability under water stress as affected by expression of SAG12-ipt controlling cytokinin synthesis in Agrostis stolonifera.Journal of experimental botany · 2011Article
- Modulation of the hormone setting by Rhodococcus fascians results in ectopic KNOX activation in Arabidopsis.Plant physiology · 2008Article
- Cytokinins in the bryophyte Physcomitrella patens: analyses of activity, distribution, and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins.Plant physiology · 2007Article
- Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors.The Biochemical journal · 2004Article
- Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity.The Plant cell · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic control of cytokinin oxidase by its substrate was investigated in planta using wild-type (WT) and conditionally ipt gene-expressing transgenic (IPT) tobacco (Nicotiana tabacum L.) callus cultures and plants. The derepression of the tetracycline (Tc)-dependent ipt gene transcription was followed by a progressive, more than 100-fold increase in total cytokinin content in IPT calli. The activity of cytokinin oxidase extracted from these calli began to increase 16 to 20 h after gene derepression, and after 13 d it was 10-fold higher than from Tc-treated WT calli. An increase in cytokinin oxidase activity, as a consequence of elevated cytokinin levels, was found in detached leaves (8-fold after 4 d) and in roots of intact plants (4-fold after 3 d). The partially purified cytokinin oxidase from WT, repressed IPT, and Tc-derepressed IPT tobacco calli exhibited similar characteristics. It had the same broad pH optimum (pH 6.5-8.5), its activity in vitro was enhanced 4-fold in the presence of copper-imidazole, and the apparent Km(N6-[[delta]2iso-pentenyl]adenine) values were in the range of 3.1 to 4.9 [mu]M. The increase in cytokinin oxidase activity in cytokinin-overproducing tissue was associated with the accumulation of a glycosylated form of the enzyme. The present data indicate the substrate induction of cytokinin oxidase activity in different tobacco tissues, which may contribute to hormone homeostasis.
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