ArticlePlant physiology1996
Characterization of Glutathione Uptake in Broad Bean Leaf Protoplasts.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 84 citations in OpenAlex.
- Biochemical and Physiological Responses of HarmfulFrontiers in microbiology · 2021Article
- Ultrasensitive magnetic resonance imaging of systemic reactive oxygen speciesChemical science · 2019Article
- Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate.Scientific reports · 2018Article
- Heat stress induces ferroptosis-like cell death in plants.The Journal of cell biology · 2017Article
- Purification and Characterization of an ATPase GsiA fromBioMed research international · 2017Article
- Compartment-specific importance of glutathione during abiotic and biotic stress.Frontiers in plant science · 2014Article
- Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion.Genetics and molecular biology · 2011Article
- Glutathione.The arabidopsis book · 2011Article
- Arabidopsis photorespiratory serine hydroxymethyltransferase activity requires the mitochondrial accumulation of ferredoxin-dependent glutamate synthase.The Plant cell · 2009Article
- Transport and sorting of the solanum tuberosum sucrose transporter SUT1 is affected by posttranslational modification.The Plant cell · 2008Article
- Localization of members of the gamma-glutamyl transpeptidase family identifies sites of glutathione and glutathione S-conjugate hydrolysis.Plant physiology · 2007Article
- Glutathione, photosynthesis and the redox regulation of stress-responsive gene expression.Photosynthesis research · 2005Review
- The yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a novel glutathione importer with an ATP-binding cassette.Journal of bacteriology · 2005Article
- A novel family of transporters mediating the transport of glutathione derivatives in plants.Plant physiology · 2004Article
- Article
- Antioxidant systems and O(2)(.-)/H(2)O(2) production in the apoplast of pea leaves. Its relation with salt-induced necrotic lesions in minor veins.Plant physiology · 2001Article
- Carrier-mediated uptake and phloem systemy of a 350-Dalton chlorinated xenobiotic with an alpha-amino acid function.Plant physiology · 2001Article
- Role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leavesPlant physiology · 1998Article
- Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis.The Plant cell · 1998Article
- Pathogen-induced changes in the antioxidant status of the apoplast in barley leavesPlant physiology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transport of reduced glutathione (GSH) and oxidized glutathione (GSSG) was studied with broad bean (Vicia faba L.) leaf tissues and protoplasts. Protoplasts and leaf discs took up GSSG at a rate about twice the uptake rate of GSH. Detailed studies with protoplasts indicated that GSH and GSSG uptake exhibited the same sensitivity to the external pH and to various chemical reagents. GSH uptake was inhibited by GSSG and glutathione conjugates. GSSG uptake was inhibited by GSH and GS conjugates, and the uptake of metolachlor-GS was inhibited by GSSG. Various amino acids (L-glutamic acid, L-glutamine, L-cysteine, L-glycine, L-methionine) and peptides (glycine-glycine, glycine-glycine-glycine) affected neither the transport of GSH nor GSSG. Uptake kinetics indicate that GSH is taken up by a single saturable transporter, with an apparent Km of 0.4 mM, whereas GSSG uptake exhibits two saturable phases, with an apparent Km of 7 [mu]M and 3.7 mM. It is concluded that the plasma membrane of leaf cells contains a specific transport system for glutathione, which takes up GSSG and GS conjugates preferentially over GSH. Proton flux measurements and electrophysiological measurements indicate that GSH and GSSG are taken up with proton symport. However, a detailed analysis of these measurements suggests that the ion movements induced by GSSG differ from those induced by GSH.
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Registered trials
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