ArticlePlant physiology1996
Specific Prenylation of Tomato Rab Proteins by Geranylgeranyl Type-II Transferase Requires a Conserved Cysteine-Cysteine Motif.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Isoprenoids and protein prenylation: implications in the pathogenesis and therapeutic intervention of Alzheimer's disease.Critical reviews in biochemistry and molecular biology · 2018Review
- Arabidopsis Rab Geranylgeranyltransferases Demonstrate Redundancy and Broad Substrate Specificity in Vitro.The Journal of biological chemistry · 2016Article
- The role of lipid post-translational modification in plant developmental processes.Frontiers in plant science · 2014Review
- Characterization, sub-cellular localization and expression profiling of the isoprenylcysteine methylesterase gene family in Arabidopsis thaliana.BMC plant biology · 2010Article
- The CaaX specificities of Arabidopsis protein prenyltransferases explain era1 and ggb phenotypes.BMC plant biology · 2010Article
- Isoprenylcysteine methylation and demethylation regulate abscisic acid signaling in Arabidopsis.The Plant cell · 2008Article
- Enlarged meristems and delayed growth in plp mutants result from lack of CaaX prenyltransferases.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Efficient prenylation by a plant geranylgeranyltransferase-I requires a functional CaaL box motif and a proximal polybasic domain.Plant physiology · 2001Article
- Functional requirement of plant farnesyltransferase during development in Arabidopsis.The Plant cell · 2000Article
- The prenylation status of a novel plant calmodulin directs plasma membrane or nuclear localization of the protein.The EMBO journal · 1999Article
- Characterization of a desiccation-responsive small GTP-binding protein (Rab2) from the desiccation-tolerant grass Sporobolus stapfianus.Plant molecular biology · 1999Article
- Protein prenylation in plants: old friends and new targets.Plant molecular biology · 1999Review
- Prenylcysteine alpha-carboxyl methyltransferase in suspension-cultured tobacco cellsPlant physiology · 1998Article
- Molecular and biochemical characterization of tomato farnesyl-protein transferase.Plant physiology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Posttranslational isoprenylation of some small GTP-binding proteins is required for their biological activity. Rab geranylgeranyl transferase (Rab GGTase) uses geranylgeranyl pyrophosphate to modify Rab proteins, its only known substrates. Geranylgeranylation of Rabs is believed to promote their association with target membranes and interaction with other proteins. Plants, like other eukaryotes, contain Rab-like proteins that are associated with intracellular membranes. However, to our knowledge, the geranylgeranylation of Rab proteins has not yet been characterized from any plant source. This report presents an activity assay that allows the characterization of prenylation of Rab-like proteins in vitro, by protein extracts prepared from plants. Tomato Rab1 proteins and mammalian Rab1a were modified by geranylgeranyl pyrophosphate but not by farnesyl pyrophosphate. This modification required a conserved cysteine-cysteine motif. A mutant form lacking the cysteine-cysteine motif could not be modified, but inhibited the geranylgeranylation of its wild-type homolog. The tomato Rab proteins were modified in vitro by protein extract prepared from yeast, but failed to become modified when the protein extract was prepared from a yeast strain containing a mutant allele for the [alpha] subunit of yeast Rab GGTase (bet4 ts). These results demonstrate that plant cells, like other eukaryotes, contain Rab GGTase-like activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.