ArticlePlant physiology1996
Polyamine Binding to Plasma Membrane Vesicles Isolated from Zucchini Hypocotyls.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
9 citing papers in PubMed, 48 citations in OpenAlex.
- Biologia futura: the role of polyamine in plant science.Biologia futura · 2020Review
- Effects of Exogenous Putrescine on Delaying Senescence of Cut Foliage ofFrontiers in plant science · 2020Article
- Polyamines regulate phosphorylation-dephosphorylation kinetics in a crustacean gill (NaMolecular and cellular biochemistry · 2017Article
- Na⁺,K⁺-ATPase activity in the posterior gills of the blue crab, Callinectes ornatus (Decapoda, Brachyura): modulation of ATP hydrolysis by the biogenic amines spermidine and spermine.The Journal of membrane biology · 2011Article
- Inhibition of cation channels in human erythrocytes by spermine.The Journal of membrane biology · 2010Article
- Spermidine-binding proteins. Purification and expression analysis in maize.Plant physiology · 2002Article
- Characterization of spermidine binding to solubilized plasma membrane proteins from zucchini hypocotylsPlant physiology · 1998Article
- Polyamines and Pectins (I. Ion Exchange and Selectivity).Plant physiology · 1997Article
- Ethylene-induced production of cadaverine is mediated by protein phosphorylation and dephosphorylation.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The general features of [14C]spermidine binding to plasmalemma vesicles isolated from zucchini (Cucurbita pepo L.) etiolated hypocotyls are reported in the present paper. The specific interaction of the polyamine with the plasma membranes was reversible and thermolabile, since it decreased by about 50% in the assay performed at 40[deg]C compared to that carried out on ice. On the contrary, nonspecific binding was unaffected by temperature. Specific spermidine binding showed a pH dependence with a maximum at pH 8.0 and it reached saturation between 0.75 and 1 mM external spermidine concentration. The value of the dissociation constant calculated from Scatchard analysis was 4.4 x 10-5 M. Specific spermidine interaction appeared to be sensitive to detergents and was markedly reduced by the presence of divalent cations, such as Mg2+ and Ca2+, whereas it was stimulated by monovalent cations. Polyamine binding sites were highly sensitive to pronase treatment. Competition experiments, performed using a series of compounds structurally related to spermidine, may provide some indication of the characteristics of spermidine binding sites. The results presented here suggest that specific spermidine binding occurs mainly with the protein component of the plasma membrane.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.