ArticleGenome biology2002
Computational discovery of sense-antisense transcription in the human and mouse genomes.
Article in Genome biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
87 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.Genome biology · 2002Pooled it
- A transcriptome-wide systematic search does not detect A-to-I RNA editing inGenome research · 2026Article
- Transcriptional interference revisited.Nature genetics · 2026Review
- Analysis of Endogenously Expressed Double-Stranded RNAs in Human Cultured Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Unraveling the Ties: Type 2 Diabetes and Parkinson's Disease - A Nano-Based Targeted Drug Delivery Approach.Current diabetes reviews · 2025Review
- Natural antisense transcripts as versatile regulators of gene expression.Nature reviews. Genetics · 2024Review
- Interdependent Transcription of a Natural Sense/Antisense Transcripts Pair (Non-coding RNA · 2022Article
- Non-coding antisense transcripts: fine regulation of gene expression in cancer.Computational and structural biotechnology journal · 2022Review
- ADAR RNA editing on antisense RNAs results in apparent U-to-C base changes on overlapping sense transcripts.Frontiers in cell and developmental biology · 2022Article
- Exon and intron sharing in opposite direction-an undocumented phenomenon in human genome-between Pou5f1 and Tcf19 genes.BMC genomics · 2021Article
- Parental bias in expression and interaction of genes in the equine placenta.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Inadvertent nucleotide sequence alterations during mutagenesis: highlighting the vulnerabilities in mouse transgenic technology.Journal, genetic engineering & biotechnology · 2021Article
- Functional roles of antisense enhancer RNA for promoting prostate cancer progression.Theranostics · 2021Article
- The internal loops in the lower stem of primary microRNA transcripts facilitate single cleavage of human Microprocessor.Nucleic acids research · 2020Article
- Article
- Analysis of the Relationship between Type II Diabetes Mellitus and Parkinson's Disease: A Systematic Review.Parkinson's disease · 2019Review
- Article
- OverGeneDB: a database of 5' end protein coding overlapping genes in human and mouse genomes.Nucleic acids research · 2018Article
- Natural Antisense Transcripts at the Interface between Host Genome and Mobile Genetic Elements.Frontiers in microbiology · 2017Article
- Development of prognostic signatures for intermediate-risk papillary thyroid cancer.BMC cancer · 2016Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOverlapping but oppositely oriented transcripts have the potential to form sense-antisense perfect double-stranded (ds) RNA duplexes. Over recent years, the number and variety of examples of mammalian gene-regulatory phenomena in which endogenous dsRNA duplexes have been proposed or demonstrated to participate has greatly increased. These include genomic imprinting, RNA interference, translational regulation, alternative splicing, X-inactivation and RNA editing. We computationally mined public mouse and human expressed sequence tag (EST) databases to search for additional examples of bidirectionally transcribed genomic regions.
resultsOur bioinformatics approach identified over 217 candidate overlapping transcriptional units, almost all of which are novel. From experimental validation of a subset of our predictions by orientation-specific RT-PCR, we estimate that our methodology has a specificity of 84% or greater. In many cases, regions of sense-antisense overlap within the 5'- or 3'-untranslated regions of a given transcript correlate with genomic patterns of mouse-human conservation.
conclusionsOur results, in conjunction with the literature, bring the total number of predicted and validated examples of overlapping but oppositely oriented transcripts to over 300. Several of these cases support the hypothesis that a subset of the instances of substantial mouse-human conservation in the 5' and 3' UTRs of transcripts might be explained in part by functionality of an overlapping transcriptional unit.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.