ArticleImmunology2002
Mechanism of induction of complement susceptibility of erythrocytes by spider and bacterial sphingomyelinases.
Article in Immunology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 88 citations in OpenAlex.
- Insights intoPathogens (Basel, Switzerland) · 2026Review
- Tetracycline reprograms inflammatory and regenerative signaling pathways in human keratinocytes exposed toFrontiers in pharmacology · 2026Article
- Role of the complement system in kidney cell death induced by Loxosceles venom Sphingomyelinases D.Archives of toxicology · 2024Article
- A Brief Overview of the Toxic Sphingomyelinase Ds of Brown Recluse Spider Venom and Other Organisms and Simple Methods To Detect Production of Its Signature Cyclic Ceramide Phosphate.Molecular pharmacology · 2024Article
- Sphingomyelinase D Activity inToxins · 2021Article
- Clinical aspects, diagnosis and management of Loxosceles spider envenomation: literature and case review.Archives of toxicology · 2020Review
- Review
- Targeting Loxosceles spider Sphingomyelinase D with small-molecule inhibitors as a potential therapeutic approach for loxoscelism.Journal of enzyme inhibition and medicinal chemistry · 2019Article
- Brown Spider (Toxins · 2019Review
- Recombinant Phospholipase D from Loxosceles gaucho Binds to Platelets and Promotes Phosphatidylserine Exposure.Toxins · 2017Article
- Phospholipase D toxins of brown spider venom convert lysophosphatidylcholine and sphingomyelin to cyclic phosphates.PloS one · 2013Article
- Complement activation by phospholipids: the interplay of factor H and C1q.Protein & cell · 2010Article
- Phospholipase D promotes Arcanobacterium haemolyticum adhesion via lipid raft remodeling and host cell death following bacterial invasion.BMC microbiology · 2010Article
- Brown recluse spider (Loxosceles reclusa) envenomation leading to acute hemolytic anemia in six adolescents.The Journal of pediatrics · 2010Article
- Sphingomyelin functions as a novel receptor for Helicobacter pylori VacA.PLoS pathogens · 2008Article
- Brown recluse spider (Loxosceles reclusa) venom phospholipase D (PLD) generates lysophosphatidic acid (LPA).The Biochemical journal · 2005Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
We have recently shown that the sphingomyelinase toxins P1 and P2 from the venom of the spider Loxosceles intermedia induce complement (C)-dependent lysis of autologous erythrocytes by induction of the cleavage of cell surface glycophorins through activation of an endogenous metalloproteinase facilitating the activation of the alternative pathway of C. Phospholipase D (PLD) from Corynebacterium pseudotuberculosis shows some degree of homology with the spider sphingomyelinases and can induce similar clinical symptoms to those observed after spider envenomation. The aim of this study was to investigate if the bacterial PLD-induced haemolysis of human erythrocytes was C dependent and if cleavage of glycophorins occurred. We show here that haemolysis of both PLD- and P1-treated human erythrocytes was C dependent, but while PLD-mediated haemolysis was dependent on activation of the classical pathway of C, P1 induced lysis via both the classical and alternative pathways. P1, but not PLD, induced cleavage of glycophorins and no change in expression of complement regulators was induced by either of the toxins. In both cases, annexin V binding sites were exposed, suggesting that the membrane asymmetry had been disturbed causing exposure of phosphatidylserine to the cell surface. Our results suggest that C susceptibility induced by L. intermedia and C. pseudotuberculosis PLD is a result of exposure of phosphatidylserine, and the higher potency of P1 toxin can be explained by its additional effect of cleavage of glycophorins.
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