ArticleGene therapy2002
Electromobility of plasmid DNA in tumor tissues during electric field-mediated gene delivery.
Article in Gene therapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 90 citations in OpenAlex.
- Importance of the electrophoresis and pulse energy for siRNA-mediated gene silencing by electroporation in differentiated primary human myotubes.Biomedical engineering online · 2024Article
- Modification of the Tumor Microenvironment Enhances Anti-PD-1 Immunotherapy in Metastatic Melanoma.Pharmaceutics · 2022Article
- The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model.Biomedical engineering online · 2021Article
- Current Progress in Electrotransfection as a Nonviral Method for Gene Delivery.Molecular pharmaceutics · 2018Article
- Efficient In Vitro Electropermeabilization of Reconstructed Human Dermal Tissue.The Journal of membrane biology · 2015Article
- New insights into the mechanisms of gene electrotransfer--experimental and theoretical analysis.Scientific reports · 2015Article
- Nonstructural protein 2 (nsP2) of Chikungunya virus (CHIKV) enhances protective immunity mediated by a CHIKV envelope protein expressing DNA Vaccine.Viral immunology · 2013Article
- In vivo molecular imaging and histological analysis of changes induced by electric pulses used for plasmid DNA electrotransfer to the skin: a study in a dorsal window chamber in mice.The Journal of membrane biology · 2012Article
- Hyaluronidase and collagenase increase the transfection efficiency of gene electrotransfer in various murine tumors.Human gene therapy · 2012Article
- Electroporation increases antitumoral efficacy of the bcl-2 antisense G3139 and chemotherapy in a human melanoma xenograft.Journal of translational medicine · 2011Article
- Efficiency of cellular delivery of antisense peptide nucleic acid by electroporation depends on charge and electroporation geometry.Oligonucleotides · 2011Article
- Enhancement of electric field-mediated gene delivery through pretreatment of tumors with a hyperosmotic mannitol solution.Cancer gene therapy · 2011Article
- Electroporation to deliver plasmid DNA into rat dental tissues.The journal of gene medicine · 2010Article
- DNA vaccines for targeting bacterial infections.Expert review of vaccines · 2010Review
- The role of electrophoresis in gene electrotransfer.The Journal of membrane biology · 2010Article
- Use of collagen gel as a three-dimensional in vitro model to study electropermeabilization and gene electrotransfer.The Journal of membrane biology · 2010Article
- Analysis and comparison of electrical pulse parameters for gene electrotransfer of two different cell lines.The Journal of membrane biology · 2010Article
- Adjuvant electrochemotherapy in veterinary patients: a model for the planning of future therapies in humans.Journal of experimental & clinical cancer research : CR · 2009Review
- Article
- Relaxin treatment of solid tumors: effects on electric field-mediated gene delivery.Molecular cancer therapeutics · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interstitial transport is a crucial step in plasmid DNA-based gene therapy. However, interstitial diffusion of large nucleic acids is prohibitively slow. Therefore, we proposed to facilitate interstitial transport of DNA via pulsed electric fields. To test the feasibility of this approach to gene delivery, we developed an ex vivo technique to quantify the magnitude of DNA movement due to pulsed electric fields in two tumor tissues: B16.F10 (a mouse melanoma) and 4T1 (a mouse mammary carcinoma). When the pulse duration and strength were 50 ms and 233 V/cm, respectively, we found that the average plasmid DNA movements per 10 pulses were 1.47 microm and 0.35 microm in B16.F10 and 4T1 tumors, respectively. The average plasmid DNA movements could be approximately tripled, ie to reach 3.69 microm and 1.01 microm, respectively, when the pulse strength was increased to 465 V/cm. The plasmid DNA mobility was correlated with the tumor collagen content, which was approximately eight times greater in 4T1 than in B16.F10 tumors. These data suggest that electric field can be a powerful driving force for improving interstitial transport of DNA during gene delivery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.