ArticlePlant physiology1997
Differential Ammonia-Elicited Changes of Cytosolic pH in Root Hair Cells of Rice and Maize as Monitored by 2[prime],7[prime]-bis-(2-Carboxyethyl)-5 (and -6)-Carboxyfluorescein-Fluorescence Ratio.
Article in Plant physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 62 citations in OpenAlex.
- Exploring the Effects ofPlants (Basel, Switzerland) · 2025Article
- Dynamics of homeostats: the basis of electrical, chemical, hydraulic, pH and calcium signaling in plants.Quantitative plant biology · 2025Review
- Cytosolic alkalinization in guard cells: an intriguing but interesting event during stomatal closure that merits further validation of its importance.Frontiers in plant science · 2024Review
- Harnessing belowground processes for sustainable intensification of agricultural systems.Plant and soil · 2022Article
- Plant nitrogen uptake and assimilation: regulation of cellular pH homeostasis.Journal of experimental botany · 2020Review
- Effects of Different Sources of Nitrogen on Endophytic Colonization of Rice Plants by Azospirillum sp. B510.Microbes and environments · 2018Article
- Regulation of Lysosomal Function by the DAF-16 Forkhead Transcription Factor Couples Reproduction to Aging inGenetics · 2017Article
- Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Rapid ammonia gas transport accounts for futile transmembrane cycling under NH3/NH4+ toxicity in plant roots.Plant physiology · 2013Article
- Article
- Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants.BMC plant biology · 2011Article
- Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis) plants.Annals of botany · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intact hair cells of young rice (Oryza sativa L.) and maize roots (Zea mays L.), grown without external nitrogen, were specifically loaded with 2[prime],7[prime]-bis-(2-carboxyethyl)-5 (and -6)-carboxyfluorescein acetoxymethyl ester to monitor fluorescence ratio cytosolic pH changes in response to external ammonia (NH4+/NH3) application. In neutral media, cytosolic pH of root hairs was 7.15 [plus or minus] 0.13 (O. sativa) and 7.08 [plus or minus] 0.11 (Z. mays). Application of 2 mM ammonia at external pH 7.0 caused a transient cytosolic alkalization (7.5 [plus or minus] 0.15 in rice; 7.23 [plus or minus] 0.13 in maize). Alkalization increased with an increase of external pH; no pH changes occurred at external pH 5.0. The influx of 13N-labeled ammonia in both plant species did not differ between external pH 5.0 and 7.0 but increased significantly with higher pH. Pretreatment with 1 mM 1-methionine sulfoximine significantly reduced the ammonia-elicited pH increase in rice but not in maize. Application of 2 mM methylammonia only caused a cytosolic pH increase at high external pH; the increase in both species compared with the ammonia-elicited alkalization in 1-methionine sulfoximine-treated roots. The differential effects indicate that cytosolic alkalization derived from (a) NH3 protonation after passive permeation of the plasma membrane and, particularly in rice, (b) additional proton consumption via the glutamine synthetase/glutamate synthase cycle.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.