ArticleArthritis research2002
Molecular profile of synovial fibroblasts in rheumatoid arthritis depends on the stage of proliferation.
Article in Arthritis research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Emerging proteoglycans and proteoglycan-targeted therapies in rheumatoid arthritis.American journal of physiology. Cell physiology · 2022Review
- Significance of a calcium-binding protein S100A14 expression in colon cancer progression.Journal of gastrointestinal oncology · 2022Article
- Diverse Roles of F-BoxProtein3 in Regulation of Various Cellular Functions.Frontiers in cell and developmental biology · 2021Review
- MiR-223/NFAT5 signaling suppresses arterial smooth muscle cell proliferation and motilityAging · 2020Article
- The Role of Calcium-Calcineurin-NFAT Signaling Pathway in Health and Autoimmune Diseases.Frontiers in immunology · 2020Review
- Role of NFAT5 in the Immune System and Pathogenesis of Autoimmune Diseases.Frontiers in immunology · 2019Review
- Oligomeric S100A4 Is Associated With Monocyte Innate Immune Memory and Bypass of Tolerance to Subsequent Stimulation With Lipopolysaccharides.Frontiers in immunology · 2019Article
- Sodium Chloride Aggravates Arthritis via Th17 Polarization.Yonsei medical journal · 2019Article
- Targeting F box protein Fbxo3 to control cytokine-driven inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- New insights on the function of SCF ubiquitin E3 ligases in the lung.Cellular signalling · 2013Review
- S100 A and B expression in normal and inflamed human limbus.Molecular vision · 2013Article
- RNA-seq analysis of synovial fibroblasts brings new insights into rheumatoid arthritis.Cell & bioscience · 2012Article
- Tonicity-independent regulation of the osmosensitive transcription factor TonEBP (NFAT5).American journal of physiology. Cell physiology · 2012Review
- Nuclear factor of activated T cells 5 regulates vascular smooth muscle cell phenotypic modulation.Arteriosclerosis, thrombosis, and vascular biology · 2011Article
- NF-AT5 is a critical regulator of inflammatory arthritis.Arthritis and rheumatism · 2011Article
- Deletion of Smad4 in fibroblasts leads to defective chondrocyte maturation and cartilage production in a TGFβ type II receptor independent manner.Biochemical and biophysical research communications · 2011Article
- Transcriptional regulation of bone and joint remodeling by NFAT.Immunological reviews · 2010Review
- Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts.Arthritis research & therapy · 2010Article
- Article
- Analyses of differential proteome of human synovial fibroblasts obtained from arthritis.Clinical rheumatology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to explore the molecular profile of proliferating rheumatoid arthritis synovial fibroblasts (RA-SF). Total RNA was extracted from two cultures of RA-SF (low-density [LD] proliferating cells and high-density [HD] nonproliferating cells) and suppression subtractive hybridization was performed to compare differential gene expression of these two cultures. Subtracted cDNA was subcloned, and nucleotide sequences were analyzed to identify each clone. Differential expression of distinct clones was confirmed by semiquantitative RT-PCR. The expression of certain genes in synovial tissues was examined by in situ hybridization. In both LD and HD cells, 44 clones were upregulated. Of the 88 total clones, 46 were identical to sequences that have previously been characterized. Twenty-nine clones were identical to cDNAs that have been identified, but with unknown functions so far, and 13 clones did not show any significant homology to sequences in GenBank (NCBI). Differential expression of distinct clones was confirmed by RT-PCR. In situ hybridization showed that certain genes, such as S100A4, NFAT5, unr and Fbx3, were also expressed predominantly in synovial tissues from patients with RA but not from normal individuals. The expression of distinct genes in proliferating RA-SF could also be found in RA synovium, suggesting that these molecules are involved in synovial activation in RA. Most importantly, the data indicate that the expression of certain genes in RA-SF depends on the stage of proliferation; therefore, the stage needs to be considered in any analysis of differential gene expression in SF.
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Registered trials
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