ArticleMolecular biology of the cell2002
Agonist-induced PIP(2) hydrolysis inhibits cortical actin dynamics: regulation at a global but not at a micrometer scale.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 97 citations in OpenAlex.
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- Molecular mechanism of GPCR spatial organization at the plasma membrane.Nature chemical biology · 2024Article
- Involvement of CaCellular and molecular neurobiology · 2023Article
- Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)PInternational journal of molecular sciences · 2021Article
- Imaging the Nanoscale Distribution of Phosphoinositides in the Cell Plasma Membrane with Single-Molecule Localization Super-Resolution Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Gαq-mediated calcium dynamics and membrane tension modulate neurite plasticity.Molecular biology of the cell · 2020Article
- Anterior-enriched filopodia create the appearance of asymmetric membrane microdomains in polarizingJournal of cell science · 2019Article
- Intracellular Domain Contacts Contribute to Ecadherin Constitutive Dimerization in the Plasma Membrane.Journal of molecular biology · 2017Article
- Skap2 is required for βThe Journal of experimental medicine · 2017Article
- Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes.Journal of visualized experiments : JoVE · 2016Article
- Cholesterol-Dependent Phase-Demixing in Lipid Bilayers as a Switch for the Activity of the Phosphoinositide-Binding Cytoskeletal Protein Gelsolin.Biochemistry · 2016Article
- Optogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Nanoscale Landscape of Phosphoinositides Revealed by Specific Pleckstrin Homology (PH) Domains Using Single-molecule Superresolution Imaging in the Plasma Membrane.The Journal of biological chemistry · 2015Article
- Atomistic Monte Carlo simulation of lipid membranes.International journal of molecular sciences · 2014Review
- Mapping the local organization of cell membranes using excitation-polarization-resolved confocal fluorescence microscopy.Biophysical journal · 2013Article
- Roles played by capsid-dependent induction of membrane curvature and Gag-ESCRT interactions in tetherin recruitment to HIV-1 assembly sites.Journal of virology · 2013Article
- Regulation of directional cell migration by membrane-induced actin bundling.Journal of cell science · 2013Article
- A spatial model for integrin clustering as a result of feedback between integrin activation and integrin binding.Biophysical journal · 2012Article
- Nonpolarized signaling reveals two distinct modes of 3D cell migration.The Journal of cell biology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylinositol 4, 5-bisphosphate (PIP(2)) at the inner leaflet of the plasma membrane has been proposed to locally regulate the actin cytoskeleton. Indeed, recent studies that use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent PIP(2) sensors suggest that this lipid is enriched in membrane microdomains. Here we report that this concept needs revision. Using three distinct fluorescent GFP-tagged pleckstrin homology domains, we show that highly mobile GFP-PH patches colocalize perfectly with various lipophilic membrane dyes and, hence, represent increased lipid content rather than PIP(2)-enriched microdomains. We show that bright patches are caused by submicroscopical folds and ruffles in the membrane that can be directly visualized at approximately 15 nm axial resolution with a novel numerically enhanced imaging method. F-actin motility is inhibited significantly by agonist-induced PIP(2) breakdown, and it resumes as soon as PIP(2) levels are back to normal. Thus, our data support a role for PIP(2) in the regulation of cortical actin, but they challenge a model in which spatial differences in PIP(2) regulation of the cytoskeleton exist at a micrometer scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.