ArticleMolecular biology of the cell2002
alpha4beta1 integrin regulates lamellipodia protrusion via a focal complex/focal adhesion-independent mechanism.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 62 citations in OpenAlex.
- Probing the protrusions: lamellipodia and filopodia in cancer invasion and beyond.Mechanobiology in medicine · 2024Review
- α4/α9 Integrins Coordinate Epithelial Cell Migration Through Local Suppression of MAP Kinase Signaling Pathways.Frontiers in cell and developmental biology · 2021Article
- Integrin α11 cytoplasmic tail is required for FAK activation to initiate 3D cell invasion and ERK-mediated cell proliferation.Scientific reports · 2019Article
- SSX2 regulates focal adhesion but does not drive the epithelial to mesenchymal transition in prostate cancer.Oncotarget · 2016Article
- Article
- Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract.Journal of visualized experiments : JoVE · 2015Article
- Integrin α4 Enhances Metastasis and May Be Associated with Poor Prognosis in MYCN-low Neuroblastoma.PloS one · 2015Article
- Distinct signaling mechanisms regulate migration in unconfined versus confined spaces.The Journal of cell biology · 2013Article
- Fibrinogen - a possible extracellular target for inositol phosphates.Messenger (Los Angeles, Calif. : Print) · 2012Article
- Knockdown of the β(1) integrin subunit reduces primary tumor growth and inhibits pancreatic cancer metastasis.International journal of cancer · 2011Article
- Association between α4 integrin cytoplasmic tail and non-muscle myosin IIA regulates cell migration.Journal of cell science · 2011Article
- Abl-interactor-1 (Abi1) has a role in cardiovascular and placental development and is a binding partner of the alpha4 integrin.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The EMILIN/Multimerin family.Frontiers in immunology · 2011Article
- alpha4 beta1-Integrin regulates directionally persistent cell migration in response to shear flow stimulation.American journal of physiology. Cell physiology · 2008Article
- Distinct FAK-Src activation events promote alpha5beta1 and alpha4beta1 integrin-stimulated neuroblastoma cell motility.Oncogene · 2008Article
- Fibulin-5 binds human smooth-muscle cells through alpha5beta1 and alpha4beta1 integrins, but does not support receptor activation.The Biochemical journal · 2007Article
- The intracellular form of human MAGP1 elicits a complex and specific transcriptional response.The international journal of biochemistry & cell biology · 2007Article
- Integrin alpha4beta1 promotes focal adhesion kinase-independent cell motility via alpha4 cytoplasmic domain-specific activation of c-Src.Molecular and cellular biology · 2005Article
- Selective modulation of integrin-mediated cell migration by distinct ADAM family members.Molecular biology of the cell · 2005Article
- Alteration of podocytes in a murine model of crescentic glomerulonephritis.Histochemistry and cell biology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
alpha4beta1 integrin plays an important role in cell migration. We show that when ectopically expressed in Chinese hamster ovary cells, alpha4beta1 is sufficient and required for promoting protrusion of broad lamellipodia in response to scratch-wounding, whereas alpha5beta1 does not have this effect. By time-lapse microscopy of cells expressing an alpha4/green fluorescent protein fusion protein, we show that alpha4beta1 forms transient puncta at the leading edge of cells that begin to protrude lamellipodia in response to scratch-wounding. The cells expressing a mutant alpha4/green fluorescent protein that binds paxillin at a reduced level had a faster response to scratch-wounding, forming alpha4-positive puncta and protruding lamellipodia much earlier. While enhancing lamellipodia protrusion, this mutation reduces random motility of the cells in Transwell assays, indicating that lamellipodia protrusion and random motility are distinct types of motile activities that are differentially regulated by interactions between alpha4beta1 and paxillin. Finally, we show that, at the leading edge, alpha4-positive puncta and paxillin-positive focal complexes/adhesions do not colocalize, but alpha4beta1 and paxillin colocalize partially in ruffles. These findings provide evidence for a specific role of alpha4beta1 in lamellipodia protrusion that is distinct from the motility-promoting functions of alpha5beta1 and other integrins that mediate cell adhesion and signaling events through focal complexes and focal adhesions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.