ArticleMolecular biology of the cell2002
All small nuclear RNAs (snRNAs) of the [U4/U6.U5] Tri-snRNP localize to nucleoli; Identification of the nucleolar localization element of U6 snRNA.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Recruitment of a splicing factor to the nuclear lamina for its inactivation.Communications biology · 2022Article
- Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq.Nucleic acids research · 2022Article
- scaRNAs and snoRNAs: Are they limited to specific classes of substrate RNAs?RNA (New York, N.Y.) · 2019Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Cajal bodies and snRNPs - friends with benefits.RNA biology · 2017Review
- Proteomic characterization of the nucleolar linker histone H1 interaction network.Journal of molecular biology · 2015Article
- Article
- Periodic expression of Sm proteins parallels formation of nuclear Cajal bodies and cytoplasmic snRNP-rich bodies.Histochemistry and cell biology · 2011Article
- An intranucleolar body associated with rDNA.Chromosoma · 2011Article
- Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C.Cellular & molecular biology letters · 2009Article
- TRF4 is involved in polyadenylation of snRNAs in Drosophila melanogaster.Molecular and cellular biology · 2008Article
- The role of the plant nucleolus in pre-mRNA processing.Current topics in microbiology and immunology · 2008Review
- Enhancement of U4/U6 small nuclear ribonucleoprotein particle association in Cajal bodies predicted by mathematical modeling.Molecular biology of the cell · 2006Article
- Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors.The Plant cell · 2006Article
- Spatial organization of transcription by RNA polymerase III.Nucleic acids research · 2006Review
- Differential subnuclear localization of RNA strands of opposite polarity derived from an autonomously replicating viroid.The Plant cell · 2003Article
- U4 snRNA nucleolar localization requires the NHPX/15.5-kD protein binding site but not Sm protein or U6 snRNA association.The Journal of cell biology · 2003Article
- Modification of Sm small nuclear RNAs occurs in the nucleoplasmic Cajal body following import from the cytoplasm.The EMBO journal · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we showed that spliceosomal U6 small nuclear RNA (snRNA) transiently passes through the nucleolus. Herein, we report that all individual snRNAs of the [U4/U6.U5] tri-snRNP localize to nucleoli, demonstrated by fluorescence microscopy of nucleolar preparations after injection of fluorescein-labeled snRNA into Xenopus oocyte nuclei. Nucleolar localization of U6 is independent from [U4/U6] snRNP formation since sites of direct interaction of U6 snRNA with U4 snRNA are not nucleolar localization elements. Among all regions in U6, the only one required for nucleolar localization is its 3' end, which associates with the La protein and subsequently during maturation of U6 is bound by Lsm proteins. This 3'-nucleolar localization element of U6 is both essential and sufficient for nucleolar localization and also required for localization to Cajal bodies. Conversion of the 3' hydroxyl of U6 snRNA to a 3' phosphate prevents association with the La protein but does not affect U6 localization to nucleoli or Cajal bodies.
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