ArticleThe Journal of cell biology2002
Agrin plays an organizing role in the formation of sympathetic synapses.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 76 citations in OpenAlex.
- The synaptic vesicle cluster as a controller of pre- and postsynaptic structure and function.The Journal of physiology · 2025Review
- Beyond the Surface: Investigating the Potential Mechanisms of Non-Motor Symptoms in Myasthenia Gravis.European journal of neurology · 2025Review
- Alpha herpesvirus exocytosis from neuron cell bodies uses constitutive secretory mechanisms, and egress and spread from axons is independent of neuronal firing activity.PLoS pathogens · 2024Article
- Heparan sulfate proteoglycan in Alzheimer's disease: aberrant expression and functions in molecular pathways related to amyloid-β metabolism.American journal of physiology. Cell physiology · 2023Review
- Heightened sympathetic neuron activity and altered cardiomyocyte properties in spontaneously hypertensive rats during the postnatal period.Frontiers in synaptic neuroscience · 2022Article
- The sympathetic nervous system in development and disease.Nature reviews. Neuroscience · 2021Review
- An Inside Job: Molecular Determinants for Postsynaptic Localization of Nicotinic Acetylcholine Receptors.Molecules (Basel, Switzerland) · 2021Review
- Possible implications of dysregulated nicotinic acetylcholine receptor diffusion and nanocluster formation in myasthenia gravis.Neural regeneration research · 2021Review
- Article
- A novel role for the extracellular matrix glycoprotein-Tenascin-X in gastric function.The Journal of physiology · 2019Article
- Single-channel properties of α3β4, α3β4α5 and α3β4β2 nicotinic acetylcholine receptors in mice lacking specific nicotinic acetylcholine receptor subunits.The Journal of physiology · 2013Article
- The role of agrin in synaptic development, plasticity and signaling in the central nervous system.Neurochemistry international · 2012Review
- LG2 agrin mutation causing severe congenital myasthenic syndrome mimics functional characteristics of non-neural (z-) agrin.Human genetics · 2012Article
- Induction of filopodia-like protrusions by transmembrane agrin: role of agrin glycosaminoglycan chains and Rho-family GTPases.Experimental cell research · 2010Article
- Engineering neuronal nicotinic acetylcholine receptors with functional sensitivity to alpha-bungarotoxin: a novel alpha3-knock-in mouse.The European journal of neuroscience · 2009Article
- Transmembrane agrin regulates dendritic filopodia and synapse formation in mature hippocampal neuron cultures.Neuroscience · 2009Article
- Target-dependent inhibition of sympathetic neuron growth via modulation of a BMP signaling pathway.Developmental biology · 2008Article
- Agrin signalling contributes to cell activation and is overexpressed in T lymphocytes from lupus patients.Journal of immunology (Baltimore, Md. : 1950) · 2007Article
- Agrin induced morphological and structural changes in growth cones of cultured hippocampal neurons.Neuroscience · 2007Article
- Synapse loss in cortex of agrin-deficient mice after genetic rescue of perinatal death.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrin is a nerve-derived factor that directs neuromuscular synapse formation, however its role in regulating interneuronal synaptogenesis is less clear. Here, we examine agrin's role in synapse formation between cholinergic preganglionic axons and sympathetic neurons in the superior cervical ganglion (SCG) using agrin-deficient mice. In dissociated cultures of SCG neurons, we found a significant decrease in the number of synapses with aggregates of presynaptic synaptophysin and postsynaptic neuronal acetylcholine receptor among agrin-deficient neurons as compared to wild-type neurons. Moreover, the levels of pre- and postsynaptic markers at the residual synapses in agrin-deficient SCG cultures were also reduced, and these defects were rescued by adding recombinant neural agrin to the cultures. Similarly, we observed a decreased matching of pre- and postsynaptic markers in SCG of agrin-deficient embryos, reflecting a decrease in the number of differentiated synapses in vivo. Finally, in electrophysiological experiments, we found that paired-pulse depression was more pronounced and posttetanic potentiation was significantly greater in agrin-deficient ganglia, indicating that synaptic transmission is also defective. Together, these findings indicate that neural agrin plays an organizing role in the formation and/or differentiation of interneuronal, cholinergic synapses.
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