ArticleJournal of bacteriology2002
Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 78 citations in OpenAlex.
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- Exometabolomic-enabled discovery of compounds associated with Escherichia coli O157:H7 population dynamics in the lettuce phyllosphere.BMC plant biology · 2026Article
- D-serine metabolism enhancesCurrent research in microbial sciences · 2026Article
- Decoupling sucrose utilization from oxygen-responsive regulation for high-efficiency L-lactic acid production in Escherichia coli.Biotechnology for biofuels and bioproducts · 2025Article
- Review
- The Scr and Csc pathways for sucrose utilization co-exist inFrontiers in microbiology · 2024Article
- Global population structure, genomic diversity and carbohydrate fermentation characteristics of clonal complex 119 (CC119), an understudied Shiga toxin-producingMicrobial genomics · 2023Article
- Construction of an artificial consortium ofFrontiers in microbiology · 2022Article
- Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L-xylulose biotransformation from xylitol.Bioprocess and biosystems engineering · 2021Article
- Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya.PLoS neglected tropical diseases · 2021Article
- Genomic plasticity of pathogenicProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Structural and Functional Adaptability of Sucrose and Lactose Permeases fromBiochemistry · 2020Article
- Adaptation of the Gut Microbiota to Modern Dietary Sugars and Sweeteners.Advances in nutrition (Bethesda, Md.) · 2020Review
- Metabolic output defines Escherichia coli as a health-promoting microbe against intestinal Pseudomonas aeruginosa.Scientific reports · 2019Article
- Metabolism of sucrose in a non-fermentative Escherichia coli under oxygen limitation.Applied microbiology and biotechnology · 2019Article
- Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution.Microbial cell factories · 2019Article
- Characterization ofOpen life sciences · 2018Article
- Article
- Electrode-assisted acetoin production in a metabolically engineeredBiotechnology for biofuels · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although Escherichia coli strain EC3132 possesses a chromosomally encoded sucrose metabolic pathway, its growth on low sucrose concentrations (5 mM) is unusually slow, with a doubling time of 20 h. In this report we describe the subcloning and further characterization of the corresponding csc genes and adjacent genes. The csc regulon comprises three genes for a sucrose permease, a fructokinase, and a sucrose hydrolase (genes cscB, cscK, and cscA, respectively). The genes are arranged in two operons and are negatively controlled at the transcriptional level by the repressor CscR. Furthermore, csc gene expression was found to be cyclic AMP-CrpA dependent. A comparison of the genomic sequences of the E. coli strains EC3132, K-12, and O157:H7 in addition to Salmonella enterica serovar Typhimurium LT2 revealed that the csc genes are located in a hot spot region for chromosomal rearrangements in enteric bacteria. The comparison further indicated that the csc genes might have been transferred relatively recently to the E. coli wild-type EC3132 at around the time when the different strains of the enteric bacteria diverged. We found evidence that a mobile genetic element, which used the gene argW for site-specific integration into the chromosome, was probably involved in this horizontal gene transfer and that the csc genes are still in the process of optimal adaptation to the new host. Selection for such adaptational mutants growing faster on low sucrose concentrations gave three different classes of mutants. One class comprised cscR(Con) mutations that expressed all csc genes constitutively. The second class constituted a cscKo operator mutation, which became inducible for csc gene expression at low sucrose concentrations. The third class was found to be a mutation in the sucrose permease that caused an increase in transport activity.
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