ArticleAmerican journal of physiology. Renal physiology2002
K depletion increases protein tyrosine kinase-mediated phosphorylation of ROMK.
Article in American journal of physiology. Renal physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Mechanistic target of rapamycin complex-1 plays a role in inhibiting kidney KAmerican journal of physiology. Renal physiology · 2026Article
- Control of sodium and potassium homeostasis by renal distal convoluted tubules.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2023Article
- Role of WNK4 and kidney-specific WNK1 in mediating the effect of high dietary KAmerican journal of physiology. Renal physiology · 2018Article
- Endoplasmic reticulum-associated degradation of the renal potassium channel, ROMK, leads to type II Bartter syndrome.The Journal of biological chemistry · 2017Article
- Src-family protein tyrosine kinase phosphorylates WNK4 and modulates its inhibitory effect on KCNJ1 (ROMK).Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Distal convoluted tubule.Comprehensive Physiology · 2015Review
- MicroRNA-194 (miR-194) regulates ROMK channel activity by targeting intersectin 1.American journal of physiology. Renal physiology · 2014Article
- Src family protein tyrosine kinase regulates the basolateral K channel in the distal convoluted tubule (DCT) by phosphorylation of KCNJ10 protein.The Journal of biological chemistry · 2013Article
- Protein phosphatase 1 modulates the inhibitory effect of With-no-Lysine kinase 4 on ROMK channels.American journal of physiology. Renal physiology · 2012Article
- Angiotensin II diminishes the effect of SGK1 on the WNK4-mediated inhibition of ROMK1 channels.Kidney international · 2011Article
- Sorting it out in endosomes: an emerging concept in renal epithelial cell transport regulation.Physiology (Bethesda, Md.) · 2010Review
- Regulation and function of potassium channels in aldosterone-sensitive distal nephron.Current opinion in nephrology and hypertension · 2010Review
- Decrease in dietary K intake stimulates the generation of superoxide anions in the kidney and inhibits K secretory channels in the CCD.American journal of physiology. Renal physiology · 2010Article
- A comprehensive guide to the ROMK potassium channel: form and function in health and disease.American journal of physiology. Renal physiology · 2009Review
- Src family protein tyrosine kinase (PTK) modulates the effect of SGK1 and WNK4 on ROMK channels.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Regulation of potassium (K) handling in the renal collecting duct.Pflugers Archiv : European journal of physiology · 2009Review
- Inhibition of angiotensin type 1 receptor impairs renal ability of K conservation in response to K restriction.American journal of physiology. Renal physiology · 2009Article
- Role of gp91phox -containing NADPH oxidase in mediating the effect of K restriction on ROMK channels and renal K excretion.Journal of the American Society of Nephrology : JASN · 2007Article
- Inhibitor of growth 4 (ING4) is up-regulated by a low K intake and suppresses renal outer medullary K channels (ROMK) by MAPK stimulation.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Mitogen-activated protein kinases inhibit the ROMK (Kir 1.1)-like small conductance K channels in the cortical collecting duct.Journal of the American Society of Nephrology : JASN · 2006Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
We purified His-tagged ROMK1 and carried out in vitro phosphorylation assays with (32)P-radiolabeled ATP to determine whether ROMK1 protein is a substrate for PTK. Addition of active c-Src and [(32)P]ATP to the purified ROMK1 protein resulted in the phosphorylation of the ROMK1 protein. However, c-Src did not phosphorylate R1Y337A in which tyrosine residue 337 was mutated to alanine. Furthermore, phosphopeptide mapping identified two phosphopeptides from the trypsin-digested ROMK1 protein. In contrast, no phosphorylated peptide has been found in the trypsin-digested R1Y337A protein. This suggested that two phosphorylated peptides might contain the same tyrosine residue. Also, addition of c-Src and [(32)P]ATP phosphorylated the synthesized peptide corresponding to amino acid sequence 333-362 of the COOH terminus of ROMK1. We then examined the effect of dietary K intake on the tyrosine-phosphorylated ROMK level. Although the ROMK channels pulled down by immunoprecipitation with ROMK antibody were the same from rats on a K-deficient diet or on a high-K diet, more ROMK channels were phosphorylated by PTK in rats on a K-deficient diet than those on a high-K diet. We conclude that ROMK1 can be phosphorylated by PTK and that tyrosine residue 337 is the key site for the phosphorylation. Also, the tyrosine phosphorylation of ROMK is modulated by dietary K intake. This strongly suggests that PTK is an important member of the aldosterone-independent signal transduction pathway for regulating renal K secretion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.