ArticleMolecular and cellular biology2002
The Ccr4-not complex and yTAF1 (yTaf(II)130p/yTaf(II)145p) show physical and functional interactions.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 69 citations in OpenAlex.
- Characterization of BioID tagging systems in budding yeast and exploring the interactome of the Ccr4-Not complex.G3 (Bethesda, Md.) · 2024Article
- Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability.Cells · 2023Article
- Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events.Genome biology · 2023Article
- Review
- Genome-Wide Mapping of Decay Factor-mRNA Interactions in Yeast Identifies Nutrient-Responsive Transcripts as Targets of the Deadenylase Ccr4.G3 (Bethesda, Md.) · 2018Article
- Def1 interacts with TFIIH and modulates RNA polymerase II transcription.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Alternative splicing of CNOT7 diversifies CCR4-NOT functions.Nucleic acids research · 2017Article
- The CCR4-NOT complex contributes to repression of Major Histocompatibility Complex class II transcription.Scientific reports · 2017Article
- Transcription factors that influence RNA polymerases I and II: To what extent is mechanism of action conserved?Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Review
- The Ccr4-Not complex is a key regulator of eukaryotic gene expression.Wiley interdisciplinary reviews. RNA · 2016Review
- The architecture of the Schizosaccharomyces pombe CCR4-NOT complex.Nature communications · 2016Article
- CNOT3 contributes to early B cell development by controlling Igh rearrangement and p53 mRNA stability.The Journal of experimental medicine · 2015Article
- Article
- The Not5 subunit of the ccr4-not complex connects transcription and translation.PLoS genetics · 2014Article
- Insights into the structure and architecture of the CCR4-NOT complex.Frontiers in genetics · 2014Review
- Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex.Nature structural & molecular biology · 2013Article
- The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control.ISRN molecular biology · 2013Review
- The cellular roles of Ccr4-NOT in model and pathogenic fungi-implications for fungal virulence.Frontiers in genetics · 2013Article
- RNA degradation in Saccharomyces cerevisae.Genetics · 2012Review
- SPT5 affects the rate of mRNA degradation and physically interacts with CCR4 but does not control mRNA deadenylation.American journal of molecular biology · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae Ccr4-Not complex is a global regulator of transcription that is thought to regulate TATA binding protein (TBP) function at certain promoters specifically. In this paper, we show interactions between the essential domain of Not1p, which interacts with Not4p and Not5p, and the N-terminal domain of yTAF1. We isolated a temperature-sensitive nonsense allele of TAF1, taf1-4, which is synthetically lethal at the permissive temperature when combined with not4 and not5 mutants and which produces high levels of a C-terminally truncated yTAF1 derivative. Overexpression of C-terminally truncated yTAF1 is toxic in not4 or not5 mutants, whereas overexpression of full-length yTAF1 suppresses not4. Furthermore, mutations in the autoinhibitory N-terminal TAND domain of yTAF1 suppress not5, and the overexpression of similar mutants does not suppress not4. We find that, like Not5p, yTAF1 acts as a repressor of stress response element-dependent transcription. Finally, we have evidence for stress-regulated occupancy of promoter DNA by Not5p and for Not5p-dependent regulation of yTAF1 association with promoter DNA. Taken together with our finding that Not1p copurifies with glutathione S-transferase-yTaf1 in large complexes, these results provide the first molecular evidence that the Ccr4-Not complex might interact with yTAF1 to regulate its association at promoters, a function that might in turn regulate the autoinhibitory N-terminal domain of yTAF1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.