ArticleThe Plant cell2002
Molecular dissection of the gibberellin/abscisic acid signaling pathways by transiently expressed RNA interference in barley aleurone cells.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 121 citations in OpenAlex.
- The F-box protein CHALK10 mediates SEMIDWARF-1 ubiquitination and degradation to negatively regulate grain chalkiness in rice.Plant communications · 2025Article
- Alterations in starch, sucrose, and abscisic acid metabolism under drought stress in contrasting genotypes of chickpea.Journal of biosciences · 2025Article
- The effect of exogenous gibberellin and its synthesis inhibitor treatments for morphological and physiological characteristics of Tartary buckwheat.Scientific reports · 2024Article
- Manipulating GA-Related Genes for Cereal Crop Improvement.International journal of molecular sciences · 2022Review
- Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA).Planta · 2022Article
- Late-maturity α-amylase (LMA): exploring the underlying mechanisms and end-use quality effects in wheat.Planta · 2021Review
- Gibberellin Metabolism and Signaling: Targets for Improving Agronomic Performance of Crops.Plant & cell physiology · 2020Review
- The GAMYB gene in rye: sequence, polymorphisms, map location, allele-specific markers, and relationship with α-amylase activity.BMC genomics · 2020Article
- Genetic Analysis and Molecular Breeding Applications of Malting Quality QTLs in Barley.Frontiers in genetics · 2019Review
- Improving biomass and starch accumulation of bioenergy crop duckweed (Landoltia punctata) by abscisic acid application.Scientific reports · 2018Article
- Molecular Mechanisms Underlying Abscisic Acid/Gibberellin Balance in the Control of Seed Dormancy and Germination in Cereals.Frontiers in plant science · 2018Review
- Transcriptome Analysis of the Effects of Shell Removal and Exogenous Gibberellin on Germination of Zanthoxylum Seeds.Scientific reports · 2017Article
- Comparative transcriptome analysis of wheat embryo and endosperm responses to ABA and H2O2 stresses during seed germination.BMC genomics · 2016Article
- Regulatory Networks in Pollen Development under Cold Stress.Frontiers in plant science · 2016Review
- A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.).Plant molecular biology · 2016Article
- RNAi: antiviral therapy against dengue virus.Asian Pacific journal of tropical biomedicine · 2013Review
- Overexpression of RoDELLA impacts the height, branching, and flowering behaviour of Pelargonium × domesticum transgenic plants.Plant cell reports · 2012Article
- Convergent starvation signals and hormone crosstalk in regulating nutrient mobilization upon germination in cereals.The Plant cell · 2012Article
- Characterization of miRNAs in response to short-term waterlogging in three inbred lines of Zea mays.PloS one · 2012Article
- H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination.Journal of experimental botany · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction between two phytohormones, gibberellins (GA) and abscisic acid (ABA), is an important factor regulating the developmental transition from seed dormancy to germination. In cereal aleurone tissue, GA induces and ABA suppresses the expression of alpha-amylases that are essential for the utilization of starch stored in the endosperm. In this work, the signaling pathways mediated by these hormones were investigated in the aleurone cells of barley seeds using double-stranded RNA interference (RNAi) technology. In this tissue, double-stranded RNA molecules generated from the transient expression of DNA templates caused a sequence-specific suppression of the target genes. We demonstrate that the transcription factor, GAMyb, is not only sufficient but also necessary for the GA induction of alpha-amylase. Another regulatory protein, SLN1, is shown to be a repressor of GA action, and the use of RNAi technology to inhibit the synthesis of SLN1 led to derepression of alpha-amylase even in the absence of GA. However, this effect still was suppressed by ABA. Although the ABA-induced Ser/Thr protein kinase, PKABA1, is known to suppress GA-induced alpha-amylase expression, PKABA1 RNAi did not hamper the inhibitory effect of ABA on the expression of alpha-amylase, indicating that a PKABA1-independent signaling pathway also may exist. We suggest that the generation of specific RNAi in a transient expression approach is a useful technique for elucidating the role of regulatory molecules in biological systems in which conventional mutational studies cannot be performed easily.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.