ArticleOncogene2002
Thioredoxin reductase regulates AP-1 activity as well as thioredoxin nuclear localization via active cysteines in response to ionizing radiation.
Article in Oncogene, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 118 citations in OpenAlex.
- Cytotoxic and Radiosensitising Effects of a Novel Thioredoxin Reductase Inhibitor in Brain Cancers.Molecular neurobiology · 2022Article
- Thioredoxin-dependent system. Application of inhibitors.Journal of enzyme inhibition and medicinal chemistry · 2021Review
- miR-125a-5p Functions as Tumor Suppressor microRNA And Is a Marker of Locoregional Recurrence And Poor prognosis in Head And Neck Cancer.Neoplasia (New York, N.Y.) · 2019Article
- Disulfide reductase systems in liver.British journal of pharmacology · 2019Review
- NADPH-dependent and -independent disulfide reductase systems.Free radical biology & medicine · 2018Review
- MiR-23-TrxR1 as a novel molecular axis in skeletal muscle differentiation.Scientific reports · 2017Article
- Dynamic proteomics reveals bimodal protein dynamics of cancer cells in response to HSP90 inhibitor.BMC systems biology · 2017Article
- HuR silencing elicits oxidative stress and DNA damage and sensitizes human triple-negative breast cancer cells to radiotherapy.Oncotarget · 2016Article
- The expression and activity of thioredoxin reductase 1 splice variants v1 and v2 regulate the expression of genes associated with differentiation and adhesion.Bioscience reports · 2015Article
- The cysteine proteome.Free radical biology & medicine · 2015Review
- Induction of Thioredoxin Reductase 1 by Korean Red Ginseng Water Extract Regulates Cytoprotective Effects on Human Endothelial Cells.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- RNA-seq data analysis at the gene and CDS levels provides a comprehensive view of transcriptome responses induced by 4-hydroxynonenal.Molecular bioSystems · 2013Article
- Oxidative stress-related transcription factors in the regulation of secondary metabolism.Toxins · 2013Review
- Annexin A2: the importance of being redox sensitive.International journal of molecular sciences · 2013Article
- Nuclear expression of thioredoxin-1 in the invasion front is associated with outcome in patients with gallbladder carcinoma.HPB : the official journal of the International Hepato Pancreato Biliary Association · 2012Article
- Effects of redox modulation by inhibition of thioredoxin reductase on radiosensitivity and gene expression.Journal of cellular and molecular medicine · 2012Article
- Evidence for a role of oxidative stress in the carcinogenicity of ochratoxin a.Journal of toxicology · 2011Article
- Generation of double-labeled reporter cell lines for studying co-dynamics of endogenous proteins in individual human cells.PloS one · 2010Article
- Redox control systems in the nucleus: mechanisms and functions.Antioxidants & redox signaling · 2010Review
- c-Jun-NH2 terminal kinase (JNK)-mediates AP-1 activation by thioredoxin: phosphorylation of cJun, JunB, and Fra-1.Molecular and cellular biochemistry · 2010Article
Corrections and comments
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Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
Abstract
A recently identified class of signaling factors uses critical cysteine motif(s) that act as redox-sensitive 'sulfhydryl switches' to reversibly modulate specific signal transduction cascades regulating downstream proteins with similar redox-sensitive sites. For example, signaling factors such as redox factor-1 (Ref-1) and transcription factors such as the AP-1 complex both contain redox-sensitive cysteine motifs that regulate activity in response to oxidative stress. The mammalian thioredoxin reductase-1 (TR) is an oxidoreductase selenocysteine-containing flavoprotein that also appears to regulate multiple downstream intracellular redox-sensitive proteins. Since ionizing radiation (IR) induces oxidative stress as well as increases AP-1 DNA-binding activity via the activation of Ref-1, the potential roles of TR and thioredoxin (TRX) in the regulation of AP-1 activity in response to IR were investigated. Permanently transfected cell lines that overexpress wild type TR demonstrated constitutive increases in AP-1 DNA-binding activity as well as AP-1-dependent reporter gene expression, relative to vector control cells. In contrast, permanently transfected cell lines expressing a TR gene with the active site cysteine motif deleted were unable to induce AP-1 activity or reporter gene expression in response to IR. Transient genetic overexpression of either the TR wild type or dominant-negative genes demonstrated similar results using a transient assay system. One mechanism through which TR regulates AP-1 activity appears to involve TRX sub-cellular localization, with no change in the total TRX content of the cell. These results identify a novel function of the TR enzyme as a signaling factor in the regulation of AP-1 activity via a cysteine motif located in the protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.