ArticleThe Journal of cell biology2002
DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- Site directed mutagenesis reveals functional importance of conserved amino acid residues within the N-terminal domain of Dpb2 in budding yeast.Archives of microbiology · 2024Article
- Article
- Organization of DNA Replication Origin Firing inGenes · 2021Article
- APE2 promotes DNA damage response pathway from a single-strand break.Nucleic acids research · 2018Article
- The Intra-S Checkpoint Responses to DNA Damage.Genes · 2017Review
- Control of DNA replication by the nucleus/cytoplasm ratio in Xenopus.The Journal of biological chemistry · 2013Article
- Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts.Nucleic acids research · 2013Article
- The DNA damage checkpoint response to replication stress: A Game of Forks.Frontiers in genetics · 2013Article
- Study of the DNA damage checkpoint using Xenopus egg extracts.Journal of visualized experiments : JoVE · 2012Article
- Role of replication protein A as sensor in activation of the S-phase checkpoint in Xenopus egg extracts.Nucleic acids research · 2012Article
- Safeguarding genome integrity: the checkpoint kinases ATR, CHK1 and WEE1 restrain CDK activity during normal DNA replication.Nucleic acids research · 2012Review
- DNA is a co-factor for its own replication in Xenopus egg extracts.Nucleic acids research · 2011Article
- ATR activation and replication fork restart are defective in FANCM-deficient cells.The EMBO journal · 2010Article
- Physical interaction between replication protein A (RPA) and MRN: involvement of RPA2 phosphorylation and the N-terminus of RPA1.Biochemistry · 2009Article
- XRCC1 interacts with the p58 subunit of DNA Pol alpha-primase and may coordinate DNA repair and replication during S phase.Nucleic acids research · 2009Article
- TopBP1 and DNA polymerase-alpha directly recruit the 9-1-1 complex to stalled DNA replication forks.The Journal of cell biology · 2009Article
- The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling.Molecular and cellular biology · 2008Article
- ATR: an essential regulator of genome integrity.Nature reviews. Molecular cell biology · 2008Review
- Silencing of endo-exonuclease expression sensitizes mouse B16F10 melanoma cells to DNA damaging agents.Investigational new drugs · 2007Article
- The structural determinants of checkpoint activation.Genes & development · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Alkylating agents, such as methyl methanesulfonate (MMS), damage DNA and activate the DNA damage checkpoint. Although many of the checkpoint proteins that transduce damage signals have been identified and characterized, the mechanism that senses the damage and activates the checkpoint is not yet understood. To address this issue for alkylation damage, we have reconstituted the checkpoint response to MMS in Xenopus egg extracts. Using four different indicators for checkpoint activation (delay on entrance into mitosis, slowing of DNA replication, phosphorylation of the Chk1 protein, and physical association of the Rad17 checkpoint protein with damaged DNA), we report that MMS-induced checkpoint activation is dependent upon entrance into S phase. Additionally, we show that the replication of damaged double-stranded DNA, and not replication of damaged single-stranded DNA, is the molecular event that activates the checkpoint. Therefore, these data provide direct evidence that replication forks are an obligate intermediate in the activation of the DNA damage checkpoint.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.