ArticleRNA (New York, N.Y.)2002
The conserved central domain of yeast U6 snRNA: importance of U2-U6 helix Ia in spliceosome assembly.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Systematic analysis of snRNA genes reveals frequent RNU2-2 variants in dominant and recessive developmental and epileptic encephalopathies.Nature genetics · 2026Article
- Epitranscriptomic sculpting: the role of mMedical oncology (Northwood, London, England) · 2025Review
- Systematic analysis of snRNA genes reveals frequentmedRxiv : the preprint server for health sciences · 2025Article
- Structural and functional modularity of the U2 snRNP in pre-mRNA splicing.Critical reviews in biochemistry and molecular biology · 2019Review
- Article
- Spliceosome assembly in the absence of stable U4/U6 RNA pairing.RNA (New York, N.Y.) · 2015Article
- Conformational heterogeneity of the protein-free human spliceosomal U2-U6 snRNA complex.RNA (New York, N.Y.) · 2013Article
- Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration.Cell · 2012Article
- Article
- Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps.RNA (New York, N.Y.) · 2009Article
- A dynamic bulge in the U6 RNA internal stem-loop functions in spliceosome assembly and activation.RNA (New York, N.Y.) · 2007Article
- Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing.The EMBO journal · 2006Article
- Free energy landscapes of RNA/RNA complexes: with applications to snRNA complexes in spliceosomes.Journal of molecular biology · 2006Article
- New tertiary constraints between the RNA components of active yeast spliceosomes: a photo-crosslinking study.RNA (New York, N.Y.) · 2004Article
- Multiple functions for the invariant AGC triad of U6 snRNA.RNA (New York, N.Y.) · 2004Article
- A conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation.RNA (New York, N.Y.) · 2002Article
- The 5' and 3' domains of yeast U6 snRNA: Lsm proteins facilitate binding of Prp24 protein to the U6 telestem region.RNA (New York, N.Y.) · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In the pre-mRNA processing machinery of eukaryotic cells, U6 snRNA is located at or near the active site for pre-mRNA splicing catalysis, and U6 is involved in catalyzing the first chemical step of splicing. We have further defined the roles of key features of yeast U6 snRNA in the splicing process. By assaying spliceosome assembly and splicing in yeast extracts, we found that mutations of yeast U6 nt 56 and 57 are similar to previously reported deletions of U2 nt 27 or 28, all within yeast U2-U6 helix Ia. These mutations lead to the accumulation of yeast A1 spliceosomes, which form just prior to the Prp2 ATPase step and the first chemical step of splicing. These results strongly suggest that, at a late stage of spliceosome assembly, the presence of U2-U6 helix Ia is important for promoting the first chemical step of splicing, presumably by bringing together the 5' splice site region of pre-mRNA, which is base paired to U6 snRNA, and the branchsite region of the intron, which is base paired to U2 snRNA, for activation of the first chemical step of splicing, as previously proposed by Madhani and Guthrie [Cell, 1992, 71: 803-817]. In the 3' intramolecular stem-loop of U6, mutation G81C causes an allele-specific accumulation of U6 snRNP. Base pairing of the U6 3' stem-loop in yeast spliceosomes does not extend as far as to include the U6 sequence of U2-U6 helix Ib, in contrast to the human U6 3' stem-loop structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.