ArticleRNA (New York, N.Y.)2002
Expression of the essential mRNA export factor Yra1p is autoregulated by a splicing-dependent mechanism.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Direct RNA-seq provides evidence for an antiterminator function of the yeast Hrp1 protein on RNA polymerase II transcripts.RNA (New York, N.Y.) · 2026Article
- Direct RNA-seq provides evidence for an antiterminator function of the yeast Hrp1 protein on RNA polymerase II transcripts.bioRxiv : the preprint server for biology · 2025Article
- Swt21p Is Required for Nam8p-U1 snRNP Association and Efficient Pre-mRNA Splicing inInternational journal of molecular sciences · 2025Article
- Transcriptome analysis of two isolates of the tomato pathogen Cladosporium fulvum, uncovers genome-wide patterns of alternative splicing during a host infection cycle.PLoS pathogens · 2024Article
- Replicative aging in yeast involves dynamic intron retention patterns associated with mRNA processing/export and protein ubiquitination.Microbial cell (Graz, Austria) · 2024Article
- The functional complexity of the RNA-binding protein Yra1: mRNA biogenesis, genome stability and DSB repair.Current genetics · 2020Review
- The mRNA export adaptor Yra1 contributes to DNA double-strand break repair through its C-box domain.PloS one · 2019Article
- Proteomic identification of histone post-translational modifications and proteins enriched at a DNA double-strand break.Nucleic acids research · 2017Article
- Novel Intronic RNA Structures Contribute to Maintenance of Phenotype in Saccharomyces cerevisiae.Genetics · 2016Article
- Excess of Yra1 RNA-Binding Factor Causes Transcription-Dependent Genome Instability, Replication Impairment and Telomere Shortening.PLoS genetics · 2016Article
- Alternative splicing acting as a bridge in evolution.Stem cell investigation · 2015Article
- Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.PloS one · 2013Article
- Cotranscriptional splicing efficiency differs dramatically between Drosophila and mouse.RNA (New York, N.Y.) · 2012Article
- Regulated pre-mRNA splicing: the ghostwriter of the eukaryotic genome.Biochimica et biophysica acta · 2012Review
- RNA secondary structure mediates alternative 3'ss selection in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2012Article
- RNA splicing and debranching viewed through analysis of RNA lariats.Molecular genetics and genomics : MGG · 2011Article
- SUS1 introns are required for efficient mRNA nuclear export in yeast.Nucleic acids research · 2011Article
- Key features of the two-intron Saccharomyces cerevisiae gene SUS1 contribute to its alternative splicing.Nucleic acids research · 2011Article
- The export factor Yra1 modulates mRNA 3' end processing.Nature structural & molecular biology · 2011Article
- Diverse environmental stresses elicit distinct responses at the level of pre-mRNA processing in yeast.RNA (New York, N.Y.) · 2011Article
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Authors and funding
3 authors.
Funding
Abstract
Recent evidence supports the idea that pre-mRNA splicing and mRNA export are mechanistically coupled. In metazoans, this process appears to be mediated by a multicomponent complex, which associates with the spliced RNA upstream of the exon-exon junction. One of these components (Aly/REF) has a homolog in the budding yeast Saccharomyces cerevisiae known as Yra1p. The YRA1 gene is essential for growth and required for mRNA export. Notably, YRA1 is one of the only approximately 5% intron-containing genes in yeast. Moreover, the YRA1 intron has several unusual features and is conserved in other budding yeast species. Previously, overexpression of intronless YRA1 was shown to be toxic. We show here that overexpression of the intron-containing gene results in increased levels of unspliced pre-mRNA but normal levels of Yra1 protein; conversely, expression of the cDNA results in increased levels of protein and accumulation of nuclear poly(A)+ RNA. Two additional lines of evidence suggest that expression of Yra1p is autoregulated: First, expression of excess Yra1p from a plasmid reduces the level of tagged, chromosomal Yra1p, and, second, this effect requires wild-type protein. Replacement of the YRA1 intron with that of other S. cerevisiae genes cannot rescue the dominant-negative growth defect of intronless YRA1. We conclude that the level of Yra1p is negatively autoregulated by a mechanism that involves splicing of its unusual intron. Tight control of the levels of Yra1p might be necessary to couple the rates of pre-mRNA splicing and mRNA export.
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