ArticleTransgenic research2002
The use of the uromodulin promoter to target production of recombinant proteins into urine of transgenic animals.
Article in Transgenic research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- CRISPR/Cas9-mediated targeted knock-in of human erythropoietin at the β-casein locus results in lactogenic hormone-responsive expression in HC11 mammary epithelial cells.Scientific reports · 2026Article
- Uromodulin and progression of IgA nephropathy.Clinical kidney journal · 2024Article
- Uromodulin: from physiology to rare and complex kidney disorders.Nature reviews. Nephrology · 2017Review
- Expression of heme oxygenase-1 in thick ascending loop of henle attenuates angiotensin II-dependent hypertension.Journal of the American Society of Nephrology : JASN · 2012Article
- Transgenic mice and their impact on kidney research.Pflugers Archiv : European journal of physiology · 2009Review
- Cell engineering and molecular pharming for biopharmaceuticals.The open medicinal chemistry journal · 2008Article
- Animal transgenesis: state of the art and applications.Journal of applied genetics · 2007Review
- Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice.BMC biotechnology · 2005Article
- Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk.Transgenic research · 2004Article
- Renal tubule-specific expression and urinary secretion of human growth hormone: a kidney-based transgenic bioreactor growth.Transgenic research · 2003Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A uromodulin promoter has been isolated, sequenced, and used to generate two sets of transgenic mice for expression of the lacZ marker gene and for production of the human recombinant erythropoietin (rhEPO) in urine. We demonstrated that the 5.6-kb fragment of the uromodulin gene containing the 3.7-kb promoter area and, both the first exon and part of the second exon, were sufficient to provide kidney-specific expression of the lacZ gene. Histological analysis of the lacZ expression pattern revealed beta-galactosidase activity specifically in the thick limb of Henle's loop. However, due to random integration of the transgene, ectopic expression was detected in some transgenic lines. Analysis of the EPO-transgenic mice showed that rhEPO was secreted into the urine of founder mice (up to 6 ng/ml). We were able to breed and analyze only two sublines with a very low expression level of rhEPO (up to 260 pg/ml). All of our transgenic mice expressing rhEPO in urine developed disease symptoms similar to polycythemia in humans. These included a considerable increase in red blood cell counts, hemoglobin concentration, and hematocrit concomitant with severe thrombocytopenia, all of which were detected in the rhEPO-expressing mice. Although our model did not prove to be beneficial for commercial production of rhEPO, we concluded that the uromodulin promoter could be useful for expression of other important therapeutic proteins into the urine of transgenic animals.
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