ArticleTransgenic research2002
General utility of the chicken betaB1-crystallin promoter to drive protein expression in lens fiber cells of transgenic mice.
Article in Transgenic research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 27 citations in OpenAlex.
- Construction and Identification of a Novel Mice Model of Microphthalmia.Translational vision science & technology · 2024Article
- Transgenesis and web resources in quail.eLife · 2020Article
- The Zeb proteins δEF1 and Sip1 may have distinct functions in lens cells following cataract surgery.Investigative ophthalmology & visual science · 2014Article
- Overexpression of human γC-crystallin 5 bp duplication disrupts lens morphology in transgenic mice.Investigative ophthalmology & visual science · 2011Article
- Dual roles for Prox1 in the regulation of the chicken betaB1-crystallin promoter.Investigative ophthalmology & visual science · 2008Article
- Noninvasive measurement of protein aggregation by mutant huntingtin fragments or alpha-synuclein in the lens.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Transgenic mouse technology has been very valuable for the study of lens fiber cells since they can not be propagated in cell culture. The targeting of transgenes to the lens has traditionally been done with the alphaA-crystallin promoter. However, while lens-specific, transgenic lines made with the alphaA-crystallin promoter express the transgene at levels 100-300-fold lower than endogenous alphaA-crystallin. Here we propose an alternative, the chicken betaB1-crystallin promoter (-432/+30). Transgenic mice made with this promoter have successfully expressed CAT, d/n m-calpain, Weel, and betaB2-crystallin mRNA at levels comparable to the endogenous betaB1-crystallin gene and no eye abnormalities such as cataracts, have resulted. All of the transgenic lines made with the chicken betaB1-crystallin promoter have expressed the transgene in the lens fiber cells, and the best lines express at levels close to endogenous betaB1-crystallin. While RNA expression is very high, only moderate protein expression has been achieved, implying that the high protein expression of the crystallins is partially controlled at the level of translation. Thus, the chicken betaB1-crystallin promoter directs high level RNA expression to lens fiber cells, which may be especially useful for the expression of ribozyme and anti-sense RNAs in addition to ectopic proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.